Gene Expression Data
Assay Details
Assay
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Reference:
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J:73288
Sonnenberg-Riethmacher E, et al., Development and degeneration of dorsal root ganglia in the absence of the HMG-domain transcription factor Sox10. Mech Dev. 2001 Dec;109(2):253-65
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3039432
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Gene symbol:
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Pou4f1
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Gene name:
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POU domain, class 4, transcription factor 1
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Probe:
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Brn-3.0 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Specimens were fixed in 1% paraformaldehyde and embedded in 2% agaorose prior to vibratome sectioning.
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Results
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Specimen
4E:
embryonic day 12.5
(more )
Specimen
4E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Agarose
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Specimen
4F:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Sox10tm1Weg/Sox10tm1Weg
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Agarose
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Structure |
Level |
Pattern |
Image |
Note |
TS20: dorsal root ganglion |
Present |
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4F
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In these mutant embryos, the positive cells were present in numbers reflecting the decreased size of the ganglia.
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