Probe:	Atf4 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Strong		4a
TS11: extraembryonic component	Present		4a

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Uniform	4b

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Strong		4c
TS12: future prosencephalon	Strong		4c
TS12: head mesenchyme	Strong		4c
TS12: embryo ectoderm	Strong		4c	Expression is in the ectoderm of the anterior head fold.

Structure	Level	Pattern	Image	Note
TS13: future brain	Strong	Regionally restricted	4d	Expression is more intense at the lateral edges from which the neural crest cells are migrating.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Strong		4e
TS14: prosencephalon	Strong		4e
TS14: 1st branchial arch	Strong		4e	Expression is in a stripe from the hindbrain down into the first branchial arch.
TS14: future hindbrain	Strong		4e	Expression is in a stripe from the hindbrain down into the first branchial arch.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present	Regionally restricted	4f	Expression is the posterior regions.
TS16: hindlimb bud	Strong	Regionally restricted	4f	Expression is the posterior regions.

Structure	Level	Pattern	Image	Note
TS12: future brain	Present		4g

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Strong	Regionally restricted	4h	Expression is in the lateral neural folds and in the migrating neural crest.
TS12: migrating neural crest	Strong	Regionally restricted	4h	Expression is in the lateral neural folds and in the migrating neural crest.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Strong	Regionally restricted	4i	Expression is in the lateral neural folds and in the migrating neural crest.
TS12: migrating neural crest	Strong	Regionally restricted	4i	Expression is in the lateral neural folds and in the migrating neural crest.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Uniform	4j	Section is at the posterior hindbrain.
TS12: trunk somite	Present	Uniform	4j

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present		4k	Expression is in migrating neural crest cells.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present		4m	Expression is in migrating neural crest cells.

Structure	Level	Pattern	Image	Note
TS14: cardiac muscle tissue	Present		4n
TS14: future spinal cord	Present	Regionally restricted	4n	Expression is uniform in posterior tail bud sections where the neural folds are still open.

Structure	Level	Pattern	Image	Note
TS14: trunk mesenchyme	Present	Regionally restricted	4o	Expression is in the body wall at the mid trunk level.

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Uniform	4p