Gene Expression Data
Assay Details
Assay
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Reference:
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J:47699
Pabst O, et al., Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Mech Dev. 1998 Apr;73(1):85-93
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3032438
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Gene symbol:
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Nkx2-2
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Gene name:
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NK2 homeobox 2
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Probe:
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Nkx2-2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4A:
embryonic day 8.0
(more )
Note: 3 somites.
Specimen
4A:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somites.
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Structure |
Level |
Pattern |
Image |
Note |
TS12: future brain |
Present |
Regionally restricted |
4A
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Expression is in a 'V'-shaped structure at the anterior aspect of the brain.
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Specimen
4C:
embryonic day 8.5
(more )
Note: 8 somites.
Specimen
4C:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 8.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future brain |
Present |
Regionally restricted |
4C
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Expression is in a 'V'-shaped structure at the anterior aspect of the brain and in a second domain at a more caudal position.
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Specimen
4E:
embryonic day 8.0
(more )
Note: 6 somites. Double labeled: blue - Nkx2-2; red - Nkx 2-9 (assay MGI:3029967).
Specimen
4E:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
6 somites. Double labeled: blue - Nkx2-2; red - Nkx 2-9 (assay MGI:3029967).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: future prosencephalon |
Present |
Regionally restricted |
4E
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The expression domain overlaps with Nkx2-9 expression.
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Specimen
4F:
embryonic day 8.0
(more )
Note: 6 somites. Double labeled: blue - Nkx2-2; red - Nkx 2-9 (assay MGI:3029967).
Specimen
4F:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
6 somites. Double labeled: blue - Nkx2-2; red - Nkx 2-9 (assay MGI:3029967).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: future prosencephalon |
Present |
Regionally restricted |
4F
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The expression domain overlaps with Nkx2-9 expression.
|
Specimen
4G:
embryonic day 8.0
(more )
Note: 6 somites. Double labeled: blue - Nkx2-2; red - Nkx 2-9 (assay MGI:3029967).
Specimen
4G:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Note:
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6 somites. Double labeled: blue - Nkx2-2; red - Nkx 2-9 (assay MGI:3029967).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: future hindbrain |
Present |
Regionally restricted |
4G
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The expression domain overlaps with Nkx2-9 expression.
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