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Badtm1Meg
Targeted Allele Detail
Summary
Symbol: Badtm1Meg
Name: BCL2-associated agonist of cell death; targeted mutation 1, Michael E Greenberg
MGI ID: MGI:3029528
Synonyms: BAD3SA
Gene: Bad  Location: Chr19:6919229-6929267 bp, + strand  Genetic Position: Chr19, 5.1 cM
Alliance: Badtm1Meg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:88139
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion
 
Mutation detailsThe sequences coding for the three regulatory serines: Ser-112, Ser-136, and Ser-155 were all altered to code for alanines while an FRT flanked PGK-neomycin resistance cassette was inserted into the 3' UTR. The neomycin gene was subsequently excised by crossing mutant mice to transgenic mice that express FlpE recombinase. Western blot analysis demonstrated that the level of protein expression was similar to that of the wild-type protein but that the protein produced could no longer be phosphorylated at the three regulatory serines. (J:88139)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Bad Mutation:  25 strains or lines available
References
Original:  J:88139 Datta SR, et al., Survival factor-mediated BAD phosphorylation raises the mitochondrial threshold for apoptosis. Dev Cell. 2002 Nov;3(5):631-43
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory