Cdkn1a Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:73917 Tsai KY, et al., ARF is not required for apoptosis in Rb mutant mouse embryos. Curr Biol. 2002 Jan 22;12(2):159-63
Assay type:	Western blot
MGI Accession ID:	MGI:3027938
Gene symbol:	Cdkn1a
Gene name:	cyclin dependent kinase inhibitor 1A

Antibody:	anti-p21
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 4A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
WT	E13.5	TS21: brain	Present	Not Specified		Not Specified
Rb-/-	E13.5	TS21: brain	Present (a)	involves: 129S2/SvPas	Rb1^tm1Tyj/Rb1^tm1Tyj	Not Specified
Rb-/-	E13.5	TS21: brain	Present (a)	involves: 129S2/SvPas	Rb1^tm1Tyj/Rb1^tm1Tyj	Not Specified
Rb/ARF	E13.5	TS21: brain	Present (a)	involves: 129S2/SvPas * 129X1/SvJ	Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs, Rb1^tm1Tyj/Rb1^tm1Tyj	Not Specified
Rb/ARF	E13.5	TS21: brain	Present (a)	involves: 129S2/SvPas * 129X1/SvJ	Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs, Rb1^tm1Tyj/Rb1^tm1Tyj	Not Specified
Rb/p53	E13.5	TS21: brain	Present	involves: 129S2/SvPas	Rb1^tm1Tyj/Rb1^tm1Tyj, Trp53^tm1Tyj/Trp53^tm1Tyj	Not Specified
ARF-/-	E13.5	TS21: brain	Present (b)	involves: 129X1/SvJ	Cdkn2a^tm1Cjs/Cdkn2a^tm1Cjs	Not Specified

Notes:
(a) Expression levels in the mutant are significantly upregulated compared to levels seen in the wild-type.
(b) Expression levels in the mutant are comparable to those seen in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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