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Gene Expression Data
Assay Details
Assay
Reference: J:63372 Ivanova T, et al., Ontogenetic expression and sex differences of aromatase and estrogen receptor-alpha/beta mRNA in the mouse hippocampus. Cell Tissue Res. 2000 May;300(2):231-7
Assay type: RT-PCR
MGI Accession ID: MGI:3027313
Gene symbol: Esr2
Gene name: estrogen receptor 2 (beta)
Probe: ERbeta-pA, ERbeta-pB
Probe preparation: labelled with digoxigenin
Visualized with: Phosphorimaging
Assay notes: Quantification of this data is provided in the figure. The RT-PCR products were run on a gel, transfered to a blot, and then hybridized using a gene specific probe (MGI:3027322). Some of this data is pictured in Fig. 2.
Results Image: 3C
 Sample Information Bands Other Sample Information
Lane AgeStructure 273 bp Amount Genetic BackgroundMutant Allele(s)Sex
E17 male E17.5 (a) TS25: hippocampus Present 2 µg; total RNA BALB/c Male
E17 female E17.5 (a) TS25: hippocampus Present 2 µg; total RNA BALB/c Female
P0 male P0 TS27: hippocampus Present 2 µg; total RNA BALB/c Male
P0 female P0 TS27: hippocampus Present 2 µg; total RNA BALB/c Female
P7 male P7 TS28: hippocampus Present (b) 2 µg; total RNA BALB/c Male
P7 female P7 TS28: hippocampus Present 2 µg; total RNA BALB/c Female
P15 male P15 TS28: hippocampus Present 2 µg; total RNA BALB/c Male
P15 female P15 TS28: hippocampus Present 2 µg; total RNA BALB/c Female
adult male P adult TS28: hippocampus Present 2 µg; total RNA BALB/c Male
adult female P adult TS28: hippocampus Present 2 µg; total RNA BALB/c Female
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression levels are higher in the male at this time point. This was also the time of peak expression in the male.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory