Gene Expression Data
Assay Details
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Assay
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Reference:
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J:87380
Camurri L, et al., Rig-1 a new member of Robo family genes exhibits distinct pattern of expression during mouse development. Gene Expr Patterns. 2004 Jan;4(1):99-103
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3026887
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Gene symbol:
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Robo1
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Gene name:
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roundabout guidance receptor 1
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Probe:
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Robo1 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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Some sectioned embryos were embedded in 30% gelatin, fixed in 4% formaldehyde and sectioned at 50 microns with a vibrotome. Other embryos were embedded in gelatin/albumin/sucrose/ and fixed with 2.5% gluteraldehyde and sectioned at 80 microns. The authors did not specify for each section which method was used.
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Results
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Specimen
4A:
embryonic day 11.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube |
Present |
Regionally restricted |
4A
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Expression is in two parallel stripes along the entire neural tube.
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Specimen
4A.1:
embryonic day 11.5
(more )
Specimen
4A.1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube lateral wall |
Present |
Regionally restricted |
4A.1
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Expression is in the most dorso-lateral part adjacent to the roof plate.
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Analysis Tools