Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45695
Duvillie B, et al., Imprinting at the mouse Ins2 locus: evidence for cis- and trans-allelic interactions. Genomics. 1998 Jan 1;47(1):52-7
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2682265
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Gene symbol:
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Ins2
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Gene name:
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insulin II
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Probe:
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Ins2-pI, Ins1/2-pD
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Assay notes:
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The Ins2 RT-PCR product was differentiated from the Ins1 product by MspI digestion followed by Southern blot analysis using 5'-32P-labeled oligonucleotide 5'-GTCCTGATAGCGGTCCGCCA-3' as probe.
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Results
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Image: 3_right panel
| E12.5
(a)
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TS20: yolk sac
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Present
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2.5 µg |
involves: 129S2/SvPas * C57BL/6 * DBA/2 |
Ins2tm2Jja/Ins2+ | Pooled |
The maternal allele is the Neo and the paternal allele is wild-type.
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| E14.5
(a)
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TS22: yolk sac
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Present
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2.5 µg |
involves: 129S2/SvPas * C57BL/6 * DBA/2 |
Ins2tm2Jja/Ins2+ | Pooled |
The maternal allele is the Neo and the paternal allele is wild-type.
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| E12.5
(a)
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TS20: yolk sac
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Absent
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2.5 µg |
involves: 129S2/SvPas * C57BL/6 * DBA/2 |
Ins2tm2Jja/Ins2+ | Pooled |
The maternal allele is wild-type and the paternal allele is Neo.
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| E14.5
(a)
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TS22: yolk sac
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Absent
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2.5 µg |
involves: 129S2/SvPas * C57BL/6 * DBA/2 |
Ins2tm2Jja/Ins2+ | Pooled |
The maternal allele is wild-type and the paternal allele is Neo.
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Notes:
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Analysis Tools