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Gene Expression Data
Assay Details
Assay
Reference: J:84599 Ferkowicz MJ, et al., CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 2003 Sep;130(18):4393-403
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2679370
Gene symbol: Itga2b
Gene name: integrin alpha 2b
Antibody: Anti-CD41
Detection system: Alexa Fluor 647
Assay notes: The primary antibody was directly conjugated to the Alexa Fluor 647 dye, which fluoresces blue.
Results
Specimen 3A & C: embryonic day 7.0 (more )
Note: Mid-streak stage. Double labeled: blue - Itga2b; green - Kdr (assay MGI:2679369).
Structure Level Pattern Image Note
TS10: yolk sac Weak Regionally restricted 3C , 3A Expression was detected at the proximal end of the yolk sac.
Specimen 3D & F: embryonic day 7.25 (more )
Note: Late-streak stage. Double labeled: blue - Itga2b; green - Kdr (assay MGI:2679369).
Structure Level Pattern Image Note
TS11: yolk sac Weak Regionally restricted 3F , 3D Expression was detected in a 5-10 cell diameter wide band of cells in the proximal portion of the yolk sac overlying the upper exocoelomic cavity.
Specimen 3G & I: embryonic day 7.25 (more )
Note: Double labeled: blue - Itga2b; green - Kdr (assay MGI:2679369).
Structure Level Pattern Image Note
TS11: extraembryonic visceral endoderm Absent 3G , 3I
TS11: yolk sac Weak Regionally restricted 3G , 3I Expression varied from weak to trace in the proximal yolk sac.
Specimen 3J: embryonic day 7.5 (more )
Note: Neural plate stage. Double labeled: blue - Itga2b; green - Kdr (assay MGI:2679369).
Structure Level Pattern Image Note
TS11: yolk sac Weak Regionally restricted 3J Expression was detected in the proximal yolk sac.
Specimen 3K & M: embryonic day 7.5 (more )
Note: Neural plate stage. Double labeled: blue - Itga2b; green - Kdr (assay MGI:2679369). The whole mount specimen was divided from proximal to distal to present the embryo in a planar fashion.
Structure Level Pattern Image Note
TS11: yolk sac Weak Regionally restricted 3K , 3M Expression was detected in a 5-10 cells wide, 1-2 cells thick band of cells that was visible around the full circumference of the yolk sac.
Specimen 3N: embryonic day 7.5 (more )
Note: Neural plate stage. Double labeled: blue - Itga2b; green - Kdr (assay MGI:2679369).
Structure Level Pattern Image Note
TS11: yolk sac Weak 3N
Specimen 4A & C: embryonic day 8.0 (more )
Note: 1 somite stage.Triple labeled: blue - Itga2b; red - Ly76 (assay MGI:2679371); green - Kdr (assay MGI:2679369).
Structure Level Pattern Image Note
TS12: yolk sac Present 4A , 4C Dimly and brightly expressing cells were observed in the yolk sac.
Specimen 4D & E & G: embryonic day 8.25 (more )
Note: 4 somite stage. Triple labeled: blue - Itga2b; red - Ly76 (assay MGI:2679371); green - Kdr (assay MGI:2679369). The whole mount specimen was divided from proximal to distal to present the embryo in a planar fashion.
Structure Level Pattern Image Note
TS13: yolk sac Present Regionally restricted 4E , 4G , 4D Dimly and brightly expressing cells were observed in the yolk sac, bright cells were present in clusters of 10-20 cells at borders between blood islands and capillary beds.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory