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Gene Expression Data
Assay Details
Assay
Reference: J:84599 Ferkowicz MJ, et al., CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 2003 Sep;130(18):4393-403
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2679369
Gene symbol: Kdr
Gene name: kinase insert domain protein receptor
Antibody: Anti-Flk1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A & B: embryonic day 7.0 (more )
Note: Mid-streak stage. Double labeled: green - Kdr; blue - Itga2b (assay MGI:2679370).
Structure Level Pattern Image Note
TS10: extraembryonic mesoderm Present Regionally restricted 3A , 3B Expression was detected in differentiating extraembryonic mesoderm cells migrating from the primitive streak into the developing yolk sac.
Specimen 3D & E: embryonic day 7.25 (more )
Note: Late-streak stage. Double labeled: green - Kdr; blue - Itga2b (assay MGI:2679370).
Structure Level Pattern Image Note
TS11: yolk sac Present Regionally restricted 3E , 3D Expression was detected in cells extending toward the distal leading edge of the yolk sac as it approached the embryo proper.
Specimen 3G & H: embryonic day 7.25 (more )
Note: Double labeled: green - Kdr; blue - Itga2b (assay MGI:2679370).
Structure Level Pattern Image Note
TS11: extraembryonic visceral endoderm Absent 3G , 3H
TS11: yolk sac Present Regionally restricted 3H , 3G Expression was detected in the proximal yolk sac.
Specimen 3J: embryonic day 7.5 (more )
Note: Neural plate stage. Double labeled: green - Kdr; blue - Itga2b (assay MGI:2679370).
Structure Level Pattern Image Note
TS11: yolk sac Present Regionally restricted 3J Expression was detected in the proximal region of the yolk sac.
Specimen 3K & L: embryonic day 7.5 (more )
Note: Neural plate stage. Double labeled: green - Kdr; blue - Itga2b (assay MGI:2679370). The whole mount specimen was divided from proximal to distal to present the embryo in a planar fashion.
Structure Level Pattern Image Note
TS11: yolk sac Present Regionally restricted 3K , 3L Expression was detected in the proximal region of the yolk sac. The level in single cells varied from bright to dim.
Specimen 3N: embryonic day 7.5 (more )
Note: Neural plate stage.Triple labeled: green - Kdr; blue - Itga2b (assay MGI:2679370); red - Ly76 (assay MGI:2679371).
Structure Level Pattern Image Note
TS11: extraembryonic visceral endoderm Absent 3N
TS11: yolk sac Present Regionally restricted 3N Highly expressing cells were detected in a layer between primitive erythroid elements and the outer endoderm layer. Expression was also detected in the proximal region of the yolk sac.
Specimen 4A: embryonic day 8.0 (more )
Note: 1 somite stage. Triple labeled: green - Kdr; blue - Itga2b (assay MGI:2679370); red - Ly76 (assay MGI:2679371). The whole mount specimen was divided from proximal to distal to present the embryo in a planar fashion.
Structure Level Pattern Image Note
TS12: yolk sac Present 4A
Specimen 4D & E & H: embryonic day 8.25 (more )
Note: 4 somite stage. Triple labeled: green - Kdr; blue - Itga2b (assay MGI:2679370); red - Ly76 (assay MGI:2679371). The whole mount specimen was divided from proximal to distal to present the embryo in a planar fashion.
Structure Level Pattern Image Note
TS13: yolk sac Present 4E , 4D , 4H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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