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Gene Expression Data
Assay Details
Assay
Reference: J:83255 Chi CL, et al., The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development. 2003 Jun;130(12):2633-44
Assay type: RNA in situ
MGI Accession ID: MGI:2677309
Gene symbol: Hoxa2
Gene name: homeobox A2
Probe: Hoxa2 probe9
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: A mixture of two in situ probes was used in this assay.
Results
Specimen 5O: embryonic day 8.0 (more )
Note: 7 somite stage embryo. Littermate control; exact genotype not specified. Also stained for Otx2 (assay MGI:2677308)
Structure Level Pattern Image Note
TS12: future hindbrain Strong Regionally restricted 5O Expression is present in rhombomere 3.

Specimen 5P: embryonic day 8.0; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. 7 somite stage embryo. Also stained for Otx2 (assay MGI:2677308)
Structure Level Pattern Image Note
TS12: future hindbrain Strong Regionally restricted 5P Expression is present in rhombomere 3.

Specimen 5W: embryonic day 8.5 (more )
Note: 12 somite stage embryo. Littermate control; exact genotype not specified. Also stained for Otx2 (assay MGI:2677308)
Structure Level Pattern Image Note
TS13: future hindbrain Strong Regionally restricted 5W Expression is present in rhombomere 3.

Specimen 5X: embryonic day 8.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. 13 somite stage embryo. Also stained for Otx2 (assay MGI:2677308)
Structure Level Pattern Image Note
TS13: future hindbrain Strong Regionally restricted 5X Expression is present in rhombomere 3.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory