Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676619
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Gene symbol:
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Fgf15
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Gene name:
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fibroblast growth factor 15
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Probe:
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Fgf15 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
7A:
embryonic day 8.5
(more  )
Note: 12 somite stage.
Specimen
7A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Strong |
Regionally restricted |
7A
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Strong expression in ventral and intermediate parts of P1/2 (diencephalon) and anterior midbrain just dorsal to Shh expression domain.
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| TS13: future prosencephalon |
Strong |
Regionally restricted |
7A
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Strong expression in ventral and intermediate parts of P1/2 (diencephalon) and anterior midbrain just dorsal to Shh expression domain.
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Specimen
7B:
embryonic day 8.5
(more )
Note: 12 somite stage.
Specimen
7B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Strong |
Regionally restricted |
7B
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Strong expression in ventral and intermediate parts of P1/2 and anterior midbrain just dorsal to Shh expression domain. No expression in dorsal regions of diencephalon and midbrain.
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| TS13: future prosencephalon |
Strong |
Regionally restricted |
7B
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Strong expression in ventral and intermediate parts of P1/2 and anterior midbrain just dorsal to Shh expression domain. No expression in dorsal regions of diencephalon and midbrain.
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| TS13: future hindbrain |
Strong |
Regionally restricted |
7B
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Strongly expressed just posterior to the midbrain-hindbrain isthmus.
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Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 8.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Absent |
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7C
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No expression observed within forebrain or midbrain; expression detected caudal to midbrain-hindbrain isthmus.
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| TS13: future prosencephalon |
Absent |
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7C
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No expression observed within forebrain or midbrain; expression detected caudal to midbrain-hindbrain isthmus.
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Specimen
7D:
embryonic day 9.0
(more )
Note: 14 somite stage.
Specimen
7D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Present |
Regionally restricted |
7D
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Ventral to dorsal expansionl in diencephalic and midbrain primordia. Expression extended dorsally, overlapping Dbx1 and Tcf4 domains; also strongly expressed just posterior to the midbrain-hindbrain isthmus.
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| TS14: future midbrain |
Present |
Regionally restricted |
7D
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Ventral to dorsal expansionl in diencephalic and midbrain primordia. Expression extended dorsally, overlapping Dbx1 and Tcf4 domains; also strongly expressed just posterior to the midbrain-hindbrain isthmus.
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Specimen
7E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somite stage.
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Specimen
7F:
embryonic day 9.0
(more )
Note: 16 somite stage.
Specimen
7F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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16 somite stage.
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Specimen
7G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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16 somite stage.
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Analysis Tools