Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79391
Fujiwara T, et al., Distinct requirements for extra-embryonic and embryonic bone morphogenetic protein 4 in the formation of the node and primitive streak and coordination of left-right asymmetry in the mouse. Development. 2002 Oct;129(20):4685-96
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676353
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal probe6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A, a:
embryonic day 8.0
(more  )
Note: 5 somite stage.
Specimen
1A, a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5 somite stage.
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Specimen
1A, b:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Bmp4tm1Blh/Bmp4tm1Blh
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somite stage.
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Specimen
1A, c:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Bmp4tm1Blh/Bmp4tm1Blh
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somite stage.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Bmp4tm1Blh/Bmp4tm1Blh
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
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In 4/18 mutant embryos, expression appeared relatively normal.
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| TS12: primitive streak |
Present |
Regionally restricted |
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In 4/18 mutant embryos, expression appeared relatively normal.
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Specimen
1A, d:
embryonic day 8.0
(more )
Note: 5 somite stage.
Specimen
1A, d:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5 somite stage.
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Specimen
1A, e and f:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Bmp4tm1Blh/Bmp4tm1Blh
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5 somite stage.
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Specimen
5A:
embryonic day 7.5
(more )
Note: Late streak stage.
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late streak stage.
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Specimen
5B:
embryonic day 7.5
(more )
Note: 0 somites.
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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5B
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Authors report symmetric expression in the node.
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Specimen
5C:
embryonic day 8.0
(more )
Note: 5 somite stage.
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
5C
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Asymmetric expression, higher on the left, in the node and left lateral plate mesoderm.
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| TS12: primitive streak |
Present |
Regionally restricted |
5C
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Asymmetric expression, higher on the left, in the node and left lateral plate mesoderm.
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Analysis Tools