Gene Expression Data
Assay Details
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Assay
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Reference:
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J:83936
Mechta-Grigoriou F, et al., c-jun regulation and function in the developing hindbrain. Dev Biol. 2003 Jun 15;258(2):419-31
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676198
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Gene symbol:
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Hoxb3
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Gene name:
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homeobox B3
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Probe:
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Hox-2.7 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 8.0; Juntm1Wag/Jun +
(more  )
Note: 3-4 somites.
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Juntm1Wag/Jun+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-4 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube |
Present |
Regionally restricted |
3A
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Expression is in the posterior neural tube witha a boundary at prospective rhombomeres 4 and 5. There is higher expression in the area of rhombomere 5.
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| TS12: posterior pro-rhombomere |
Present |
Regionally restricted |
3A
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Expression is in the posterior neural tube witha a boundary at prospective rhombomeres 4 and 5. There is higher expression in the area of rhombomere 5.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Juntm1Wag/Juntm1Wag
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-4 somites.
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Specimen
3C:
embryonic day 8.0; Juntm1Wag/Jun +
(more )
Note: 5-6 somites.
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Juntm1Wag/Jun+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5-6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube |
Present |
Regionally restricted |
3C
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Expression is in the posterior neural tube witha a boundary at prospective rhombomeres 4 and 5. There is higher expression in the area of rhombomere 5.
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| TS12: posterior pro-rhombomere |
Present |
Regionally restricted |
3C
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Expression is in the posterior neural tube witha a boundary at prospective rhombomeres 4 and 5. There is higher expression in the area of rhombomere 5.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Juntm1Wag/Juntm1Wag
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5-6 somites.
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Specimen
3E:
embryonic day 8.5; Juntm1Wag/Jun +
(more )
Note: 9-10 somites.
Specimen
3E:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Juntm1Wag/Jun+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9-10 somites.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Juntm1Wag/Juntm1Wag
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9-10 somites.
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Specimen
3G:
embryonic day 9.0; Juntm1Wag/Jun +
(more )
Note: 15-16 somites.
Specimen
3G:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Juntm1Wag/Jun+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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15-16 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: rhombomere 5 |
Present |
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3G
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Juntm1Wag/Juntm1Wag
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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15-16 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: rhombomere 5 |
Present |
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3H
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Expression in this mutant is the same as in wild-type embryos.
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Analysis Tools