Mbp RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:15845 Mathisen PM, et al., Identification of an embryonic isoform of myelin basic protein that is expressed widely in the mouse embryo. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10125-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:2673251
Gene symbol:	Mbp
Gene name:	myelin basic protein

Probe:	pro3, term
Probe preparation:	labelled with P32
Visualized with:	Autoradiography

Results

Image: 2 ©

	Sample Information		Bands							Other Sample Information
Lane	Age	Structure	21 kDa (b)	19.7 kDa (c)	18.5 kDa (b)	17.3 kDa (d)	17.2 kDa (b)	14 kDa (b)	Size Not Specified (e)	Amount	Genetic Background	Mutant Allele(s)	Sex
E14	E14.5 (a)	TS22: brain	Present	Absent	Absent	Present	Absent	Present	Present	1 µg; total RNA	BALB/c		Not Specified
E18	E18.5 (a)	TS26: brain	Present	Absent	Absent	Present	Absent	Present	Present	1 µg; total RNA	BALB/c		Not Specified
P0	P0	TS27: brain	Present	Present	Present	Present	Present	Present	Present	1 µg; total RNA	BALB/c		Not Specified
P6	P6	TS28: brain	Present	Present	Present	Present	Present	Present	Present	1 µg; total RNA	BALB/c		Not Specified
P10	P10	TS28: brain	Present	Present	Present	Present	Present	Present	Present	1 µg; total RNA	BALB/c		Not Specified
P18	P18	TS28: brain	Present	Absent	Present	Absent	Present	Present	Trace	1 µg; total RNA	BALB/c		Not Specified
P30	P30	TS28: brain	Present	Absent	Present	Absent	Present	Present	Absent	1 µg; total RNA	BALB/c		Not Specified
P60	P60	TS28: brain	Present	Absent	Present	Absent	Present	Present	Absent	1 µg; total RNA	BALB/c		Not Specified
P90	P90	TS28: brain	Present	Absent	Present	Absent	Present	Present	Absent	1 µg; total RNA	BALB/c		Not Specified
P162	P162	TS28: brain	Present	Absent	Present	Absent	Present	Present	Absent	1 µg; total RNA	BALB/c		Not Specified
P18 control, No RT	Control

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression levels started low at various developmental stages and then increased to peak levels at about P18. This was followed by decreasing levels to an average of about 50% to a steady-state level where the expression patterns remained constant.
(c) This was a minor transcript. Expression levels started low at various developmental stages and then increased to peak levels at about P10 and were undetectable by P18.
(d) Expression levels started low at various developmental stages and then increased to peak levels at about P10 and were undetectable by P18.
(e) This is referred to as the embryonic transcript, E-MBP.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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