Zfp125 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:54688 Giorgi S, et al., Isolation and characterization of the murine zinc finger coding gene, ZT2: expression in normal and transformed myogenic cells. Gene. 1999 Apr 1;230(1):81-90
Assay type:	RT-PCR
MGI Accession ID:	MGI:2669292
Gene symbol:	Zfp125
Gene name:	zinc finger protein 125

Probe:	ZT2-oligo A, ZT2-oligo B
Probe preparation:	DNA

Results

Image: 7D

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	270 bp (b)	180 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
L13	E13.0 (a)	TS21: limb	Present	Present	1 µg; total RNA	Not Specified		Not Specified
L17	E17.0 (a)	TS25: limb	Present	Present	1 µg; total RNA	Not Specified		Not Specified
nM	P newborn	TS27: skeletal muscle	Present	Present	1 µg; total RNA	Not Specified		Not Specified
aM	P adult	TS28: skeletal muscle	Present	Present	1 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors state this band is the result of an inappropriate annealing of the primer to the subsequent domain (with a mismatch of two nucleotides), resulting in a 264 bp product.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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