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Gene Expression Data
Assay Details
Assay
Reference: J:47668 Caspary T, et al., Multiple mechanisms regulate imprinting of the mouse distal chromosome 7 gene cluster. Mol Cell Biol. 1998 Jun;18(6):3466-74
Assay type: RT-PCR
MGI Accession ID: MGI:2664900
Gene symbol: Ascl2
Gene name: achaete-scute family bHLH transcription factor 2
Probe: Mash2-pA, Mash2-pB
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The D strain is 129S4/SvJae and the S strain is BTBR.SPR-(H19-Cdkn1c).The probe used in this assay was 5' labelled.
Results Image: 5Mash2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
D X S +/+ E9.5 (a) TS15: extraembryonic component Present Absent Not Specified; total RNA involves: 129S4/SvJae * BTBR.SPR-(H19-Cdkn1c) Not Specified
D X S -/- E9.5 (a) TS15: extraembryonic component Present Absent Not Specified; total RNA involves: 129S4/SvJae * BTBR.SPR-(H19-Cdkn1c) Dnmt1tm1Enl/Dnmt1tm1EnlNot Specified
S X D +/+ E9.5 (a) TS15: extraembryonic component Moderate Strong Not Specified; total RNA involves: 129S4/SvJae * BTBR.SPR-(H19-Cdkn1c) Not Specified
S X D -/- E9.5 (a) TS15: extraembryonic component Moderate Moderate Not Specified; total RNA involves: 129S4/SvJae * BTBR.SPR-(H19-Cdkn1c) Dnmt1tm1Enl/Dnmt1tm1EnlNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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