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Gene Expression Data
Assay Details
Assay
Reference: J:47668 Caspary T, et al., Multiple mechanisms regulate imprinting of the mouse distal chromosome 7 gene cluster. Mol Cell Biol. 1998 Jun;18(6):3466-74
Assay type: RT-PCR
MGI Accession ID: MGI:2664811
Gene symbol: Ascl2
Gene name: achaete-scute family bHLH transcription factor 2
Probe: Mash2-pA, Mash2-pB
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The probe used in this assay was 5' labelled.
Results Image: 3Mash2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
D E12.5 (a) TS20: embryo Present Absent Not Specified; total RNA 129/Sv Not Specified
S E12.5 (a) TS20: embryo Absent Present Not Specified; total RNA 129/Sv Not Specified
mat +/+ E12.5 (a) TS20: embryo Present Absent Not Specified; total RNA involves: 129S6/SvEvTac * BTBR.SPR-(H19-Cdkn1c) Not Specified
mat +/- E12.5 (a) TS20: embryo Present Absent Not Specified; total RNA involves: 129S/SvEv H19tm1Tilg/H19+Not Specified maternally inherited mutation
pat +/+ E12.5 (a) TS20: embryo Absent Present Not Specified; total RNA involves: 129S6/SvEvTac * BTBR.SPR-(H19-Cdkn1c) Not Specified
pat +/- E12.5 (a) TS20: embryo Absent Present Not Specified; total RNA involves: 129S/SvEv H19tm1Tilg/H19+Not Specified paternally inherited mutation
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This band is from the D strain.
(c) This band is from the S strain.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory