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Gene Expression Data
Assay Details
Assay
Reference: J:47668 Caspary T, et al., Multiple mechanisms regulate imprinting of the mouse distal chromosome 7 gene cluster. Mol Cell Biol. 1998 Jun;18(6):3466-74
Assay type: RT-PCR
MGI Accession ID: MGI:2664763
Gene symbol: Ascl2
Gene name: achaete-scute family bHLH transcription factor 2
Probe: Mash2-pA, Mash2-pB
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The probe used in this assay was 5' labelled.
Results Image: 2C
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
1 E12.5 (a) TS28: placenta Present Absent Not Specified; total RNA 129/Sv Not Specified This is designated strain D.
2 E12.5 (a) TS28: placenta Absent Present Not Specified; total RNA BTBR.SPR-(H19-Cdkn1c) Not Specified This is designated strain S.
3 E6.5 (a) TS9: conceptus
TS28: decidua 		Absent Absent Not Specified; total RNA involves: 129/Sv * BTBR.SPR-(H19-Cdkn1c) Not Specified This lane represents a D X S cross.
4 E7.5 (a) TS11: conceptus
TS28: decidua 		Present Absent Not Specified; total RNA involves: 129/Sv * BTBR.SPR-(H19-Cdkn1c) Not Specified This lane represents a D X S cross.
5 E8.5 (a) TS13: conceptus
TS28: decidua 		Present Absent Not Specified; total RNA involves: 129/Sv * BTBR.SPR-(H19-Cdkn1c) Not Specified This lane represents a D X S cross.
6 E9.5 (a) TS15: extraembryonic component Present Absent Not Specified; total RNA involves: 129/Sv * BTBR.SPR-(H19-Cdkn1c) Not Specified This lane represents a D X S cross.
7 E12.5 (a) TS28: placenta Present Absent Not Specified; total RNA involves: 129/Sv * BTBR.SPR-(H19-Cdkn1c) Not Specified This lane represents a D X S cross.
8 E8.5 (a) TS13: conceptus
TS28: decidua 		Present Present Not Specified; total RNA involves: 129/Sv * BTBR.SPR-(H19-Cdkn1c) Not Specified This lane represents a S X D cross.
9 E9.5 (a) TS15: extraembryonic component Absent Present Not Specified; total RNA involves: 129/Sv * BTBR.SPR-(H19-Cdkn1c) Not Specified This lane represents a S X D cross.
10 E12.5 (a) TS28: placenta Absent Present Not Specified; total RNA involves: 129/Sv * BTBR.SPR-(H19-Cdkn1c) Not Specified This lane represents a S X D cross.
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This band is from the D strain.
(c) This band is from the S strain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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