E2f6 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:63376 Kusek JC, et al., Expression of the E2F family of transcription factors during murine development. Int J Dev Biol. 2000 Apr;44(3):267-77
Assay type:	Western blot
MGI Accession ID:	MGI:2662180
Gene symbol:	E2f6
Gene name:	E2F transcription factor 6

Antibody:	Anti-E2F-6
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Control experiments indicate that one band (indicated by an arrowhead in the figure) is non-specific.

Results

Image: 7C. Individual Organs

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	28 kDa (a)	Genetic Background	Mutant Allele(s)	Sex
Heart Adult	P adult	TS28: heart	Absent	ICR		Not Specified
Heart GD 12	E12.5 (b)	TS20: heart	Present (c)	ICR		Not Specified
Liver Adult	P adult	TS28: liver	Present	ICR		Not Specified
Liver GD 12	E12.5 (b)	TS20: liver	Present	ICR		Not Specified
Tongue Adult	P adult	TS28: tongue	Absent	ICR		Not Specified
Tongue GD 12	E12.5 (b)	TS20: tongue	Present	ICR		Not Specified
Brain Adult	P adult	TS28: brain	Trace	ICR		Not Specified
Brain GD 12	E12.5 (b)	TS20: brain	Present	ICR		Not Specified

Notes:
(a) Liver, brain and tongue had the highest levels of expression at E12.5.
(b) Age normalized so that noon of plug day = E0.5.
(c) This tissue had the lowest level of expression at E12.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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