Gene Expression Data
Assay Details
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Assay
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Reference:
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J:40148
Hendriksen PJ, et al., Testis-specific expression of a functional retroposon encoding glucose-6-phosphate dehydrogenase in the mouse. Genomics. 1997 May 1;41(3):350-9
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2657192
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Gene symbol:
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G6pd2
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Gene name:
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glucose-6-phosphate dehydrogenase 2
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Probe:
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G6pd-pA, G6pd-pB
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Visualized with:
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Ethidium bromide
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Assay notes:
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The authors used primers that amplified both G6pdx and G6pd2. In the HaeIII-digested samples, the 166bp G6pdx product is cleaved into 100bp and 66bp bands (these data are shown in assay MGI:2657205). A 166bp band in these lanes reflects the presence of the uncleavable G6pd2 product. This assay was to determine the start of transcription.
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Results
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Image: 2B
| Lane not used in this assay |
| P14
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TS28: testis
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Absent
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Not Specified |
either: CD-1 or Swiss |
| Male |
HaeIII digested sample.
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| Lane not used in this assay |
| P18
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TS28: testis
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Absent
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Not Specified |
either: CD-1 or Swiss |
| Male |
HaeIII digested sample.
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| Lane not used in this assay |
| P22
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TS28: testis
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Absent
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Not Specified |
either: CD-1 or Swiss |
| Male |
Transcription starts between postnatal day 22 and 26. HaeIII digested sample.
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| Lane not used in this assay |
| P26
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TS28: testis
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Present
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Not Specified |
either: CD-1 or Swiss |
| Male |
Transcription starts between postnatal day 22 and 26. HaeIII digested sample.
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| Lane not used in this assay |
| P32
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TS28: testis
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Present
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Not Specified |
either: CD-1 or Swiss |
| Male |
HaeIII digested sample.
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