Itm2a Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:75158 Barber TD, et al., Identification of Target Genes Regulated by PAX3 and PAX3--FKHR in Embryogenesis and Alveolar Rhabdomyosarcoma. Genomics. 2002 Mar;79(3):278-84
Assay type:	Northern blot
MGI Accession ID:	MGI:2656349
Gene symbol:	Itm2a
Gene name:	integral membrane protein 2A

Probe:	Itm2a cDNA2
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging
Assay notes:	The authors examined expression in two Pax3 alleles but did not specify which was used in the pictured blot.

Results

Image: 3D

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
9.5, +	E9.5 (a)	TS15: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
9.5, -	E9.5 (a)	TS15: embryo	Present (b)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified
10.5, +	E10.5 (a)	TS17: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
10.5, -	E10.5 (a)	TS17: embryo	Present (c)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified
11.5, +	E11.5 (a)	TS19: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
11.5, -	E11.5 (a)	TS19: embryo	Present (c)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified
12.5, +	E12.5 (a)	TS20: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
12.5, -	E12.5 (a)	TS20: embryo	Present (c)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified
13, +	E13.0 (a)	TS21: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
13, -	E13.0 (a)	TS21: embryo	Present (c)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified
13.5, +	E13.5 (a)	TS21: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
13.5, -	E13.5 (a)	TS21: embryo	Present (c)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified
15.5, +	E15.5 (a)	TS23: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
15.5, -	E15.5 (a)	TS23: embryo	Present (c)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified
16.5, +	E16.5 (a)	TS24: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
16.5, -	E16.5 (a)	TS24: embryo	Present (c)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified
17.5, +	E17.5 (a)	TS25: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
17.5, -	E17.5 (a)	TS25: embryo	Present (c)	5 µg; total RNA	involves: C57BL/6J	Pax3^Sp-d/Pax3^Sp-d	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression in this mutant was the same as in wild-type embryos.
(c) Expression in this mutant was decreased compared to wild-type embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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