Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50754
Karolczak M, et al., Developmental sex differences in estrogen receptor-beta mRNA expression in the mouse hypothalamus/preoptic region. Neuroendocrinology. 1998 Oct;68(4):229-34
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2655811
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Gene symbol:
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Esr2
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Gene name:
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estrogen receptor 2 (beta)
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Probe:
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ERbeta-pA, ERbeta-pB
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Probe preparation:
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labelled with digoxigenin
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Visualized with:
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Phosphorimaging
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Assay notes:
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After amplification, the products were Southern blotted using a 30-mer. Quantification of the results of this assay are provided in Fig. 3. The probe used in this assay was 3' labelled.
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Results
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Image: 2A
| E15.0
(a)
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TS23: diencephalon
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Present
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Not Specified; total RNA |
BALB/c |
| Male |
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| E15.0
(a)
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TS23: diencephalon
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Present
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Not Specified; total RNA |
BALB/c |
| Female |
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| E17.0
(a)
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TS25: preoptic area
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Present
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Not Specified; total RNA |
BALB/c |
| Male |
hypothalamus/preoptic region (Hyp/POA)
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| E17.0
(a)
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TS25: preoptic area
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Present
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Not Specified; total RNA |
BALB/c |
| Female |
hypothalamus/preoptic region (Hyp/POA)
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| P0
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TS27: preoptic area
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Strong
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Not Specified; total RNA |
BALB/c |
| Male |
hypothalamus/preoptic region (Hyp/POA)
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| P0
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TS27: preoptic area
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Present
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Not Specified; total RNA |
BALB/c |
| Female |
hypothalamus/preoptic region (Hyp/POA)
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| P10
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TS28: preoptic area
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Strong
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Not Specified; total RNA |
BALB/c |
| Male |
hypothalamus/preoptic region (Hyp/POA)
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| P10
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TS28: preoptic area
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Present
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Not Specified; total RNA |
BALB/c |
| Female |
hypothalamus/preoptic region (Hyp/POA)
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| P15
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TS28: preoptic area
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Strong
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Not Specified; total RNA |
BALB/c |
| Male |
hypothalamus/preoptic region (Hyp/POA)
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| P15
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TS28: preoptic area
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Present
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Not Specified; total RNA |
BALB/c |
| Female |
hypothalamus/preoptic region (Hyp/POA)
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Notes:
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Analysis Tools