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Tg(Mt1-B4galt1)06Shur
Transgene Detail
Summary
Symbol: Tg(Mt1-B4galt1)06Shur
Name: transgene insertion 06, Barry D Shur
MGI ID: MGI:2388614
Transgene: Tg(Mt1-B4galt1)06Shur  Location: unknown  
Alliance: Tg(Mt1-B4galt1)06Shur page
Transgene
origin
Strain of Origin:  (C57BL/6 x DBA/2)F2
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Mt1-B4galt1)06Shur expresses 1 gene
 
Tg(Mt1-B4galt1)06Shur expression driven by 1 gene
 
Mutation details: The transgene is composed of a cDNA encoding the long form of murine B4galt1, the mouse Mt1 promoter, and human growth hormone 3' polyadenylation sequences. Intron 4 was included in the B4galt1 sequence to increase transgene expression. Expression of long form B4galt1 was verified by RNase protection assay of testes RNA. Transgene expression was also confirmed by enzyme assays, immunoprecipitation enzyme activity, and Western blot analysis. (J:20319)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Notes
Transgenic founders designated 06 and 09 are high-expressing lines and were selected for study. Enzyme activity is present in a variety of tissues examined. and activity is estimated to be 10- to 12-fold higher in testes of transgenic animals compared to wild-type. Induction with zinc increased expression of the transgene only slightly.
References
Original:  J:20319 Youakim A, et al., Overexpressing sperm surface beta 1,4-galactosyltransferase in transgenic mice affects multiple aspects of sperm-egg interactions. J Cell Biol. 1994 Sep;126(6):1573-83
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory