Probe:	mdkk-1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS14: future diencephalon	Present		1A
TS14: trunk unsegmented mesenchyme	Present		1A

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted	1C , 1B	Expression is in a thin stripe of lateral plate mesenchyme cells at the very proximal margin of the forelimb bud. A similar band can be seen at the presumptive postition of the hindlimb.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	1D	There are two domains, one at the very anterior proximal margin and another, stronger domain in the posterior part.

Structure	Level	Pattern	Image	Note
TS19: future arm mesenchyme	Present	Regionally restricted	1E	Expression is in the poserior and in the flank.

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region mesenchyme	Present		1F	It is not specified whether this ia a handplate or a footplate.

Structure	Level	Pattern	Image	Note
TS21: limb interdigital region mesenchyme	Present		1G

Structure	Level	Pattern	Image	Note
TS22: limb interdigital region	Present	Regionally restricted	1H	Expression is restricted to the interdigital mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	2B , 2A	Expression overlaps with Shh expression (see assay MGI:2388203).

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2C	The expression domain is proximally restricted and separate from Shh expression (see assay MGI:2388203).

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present	Regionally restricted		Expression remains distinct from that of Shh (see assay MGI:2388203).