Tg(KRT14-Prkca)115Akv
Transgene Detail
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| Symbol: |
Tg(KRT14-Prkca)115Akv |
| Name: |
transgene insertion 115, Ajit K Verma |
| MGI ID: |
MGI:2385710 |
| Synonyms: |
alpha-115 |
| Transgene: |
Tg(KRT14-Prkca)115Akv Location: unknown
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| Alliance: |
Tg(KRT14-Prkca)115Akv page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(KRT14-Prkca)115Akv expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Prkca (MGI:97595) |
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Mutation details: A mouse Prkca cDNA tagged with bacteriophage T7 major coat protein at the N-terminus, and under the control of the human keratin 14 promoter, was used as the transgene. Human keratin 14 promoter sequences direct expression of the transgene in the basal epithelium. Western blot also detected expression of the transgene in thymus, ear, and eyeball of transgenic animals. Expression of the transgene was 8X that of endogenous protein as determined by Western blot analysis. Kinase activity of the transgene product was confirmed by PKC immunocomplex kinase assay.
(J:70923)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Founder 115 carries 90 integrated copies of the transgene and exhibits a high level of protein expression and activity.
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| Original: |
J:70923 Jansen AP, et al., Relation of the induction of epidermal ornithine decarboxylase and hyperplasia to the different skin tumor-promotion susceptibilities of protein kinase C alpha, -delta and -epsilon transgenic mice. Int J Cancer. 2001 Sep 1;93(5):635-43 |
| All: |
2 reference(s) |
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Analysis Tools
