Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Per3tm1Drw
Name:	period circadian clock 3; targeted mutation 1, David R Weaver
MGI ID:	MGI:2385797
Synonyms:	mPER3-deficient
Gene:	Per3  Location: Chr4:151088109-151129122 bp, - strand  Genetic Position: Chr4, 81.53 cM
Alliance:	Per3tm1Drw page

Germline Transmission:	Earliest citation of germline transmission: J:63933
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The gene was disrupted by replacement of exon 3 and part of exon 4 (encoding amino acids 92-154) with a PGK-neo cassette via homologous recombination. Introduction of PGK-neo introduces stop codons into all 3 reading frames. Western blot analysis of brain lysates from homozygous mutant animals using antibodies directed against the C-terminus of the protein confirmed the absence of protein expression. Immunoprecipitation assays also confirmed the absence of protein product. Northern blot and RT-PCR analysis detected mutant transcripts containing neo and lacking exon 3 and part of exon 4 in brain and skeletal muscles from homozygous mutants. (J:63933)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Per3 Mutation:	47 strains or lines available

Original:	J:63933 Shearman LP, et al., Targeted disruption of the mPer3 gene: subtle effects on circadian clock function. Mol Cell Biol. 2000 Sep;20(17):6269-75
All:	27 reference(s)