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Gene Expression Data
Assay Details
Assay
Reference: J:76131 Holley SJ, et al., Complementary Expression of IGF-II and IGFBP-5 during Anterior Pituitary Development. Dev Biol. 2002 Apr 15;244(2):319-28
Assay type: RNA in situ
MGI Accession ID: MGI:2179265
Gene symbol: Igfbp5
Gene name: insulin-like growth factor binding protein 5
Probe: Igfbp5 cDNA4
Probe preparation: Antisense RNA
Assay notes: Probes were labeled with 32P or 35S.
Results
Specimen E10.5: embryonic day 10.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS17: oral epithelium Present 5_IGFBP5 e10.5
TS17: Rathke's pouch Absent 5_IGFBP5 e10.5
Specimen E11.5: embryonic day 11.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS19: Rathke's pouch Strong Regionally restricted 5_IGFBP5 e11.5 Expression in the ventral epithelium.
Specimen E12.5: embryonic day 12.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS20: remnant of Rathke's pouch Present Regionally restricted 5_IGFBP5 e12.5 Expression in the rostral region and in the ventral region pouch epithelium.
Specimen E14.5: embryonic day 14.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS22: pituitary gland Strong 6_IGFBP5 e14.5 Expression is seen rostral to the anterior pituitary.
TS22: adenohypophysis Weak 6_IGFBP5 e14.5
Specimen E18.5: embryonic day 18.5 (more )
Note: Embryos were fixed in neutral-buffered formalin for 4 days with 8% sucrose added on the last day.
Structure Level Pattern Image Note
TS26: adenohypophysis Present Regionally restricted 6_IGFBP5 e18.5 High expression in the rostral tip of the anterior pituitary, and faint and spotty expression in the rest of the anterior pituitary.
TS26: neurohypophysis Weak Regionally restricted 6_IGFBP5 e18.5 Expression in the intermediate and posterior pituitary.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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