Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74236
Fu H, et al., Dual origin of spinal oligodendrocyte progenitors and evidence for the cooperative role of Olig2 and Nkx2.2 in the control of oligodendrocyte differentiation. Development. 2002 Feb;129(3):681-93
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2176523
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Gene symbol:
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Pdgfra
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Gene name:
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platelet derived growth factor receptor, alpha polypeptide
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Probe:
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Pdgfra cDNA11
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4D:
embryonic day 12.5
(more  )
Note: Double labeled: blue - Pdgfra; brown - Olig2 (assay MGI:2176469).
Specimen
4D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Pdgfra; brown - Olig2 (assay MGI:2176469).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4D
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Expression detected in the ventral neuroepithelium of the spinal cord adjacent to or within Olig2 positive cells.
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Specimen
4E:
embryonic day 12.5
(more )
Note: Double labeled: blue - Pdgfra; brown - Olig2 (assay MGI:2176469).
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Pdgfra; brown - Olig2 (assay MGI:2176469).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4E
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Expression detected in the ventral neuroepithelium of the spinal cord adjacent to or within Olig2 positive cells and is the same as in wild type.
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Specimen
4F:
embryonic day 12.5
(more )
Note: Double labeled: blue - Pdgfra; brown - Nkx2-2 (assay MGI:2176510).
Specimen
4F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Pdgfra; brown - Nkx2-2 (assay MGI:2176510).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4F
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Expression detected in the ventral neuroepithelium of the spinal cord dorsal to Nkx2-2 positive cells.
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Specimen
7A:
embryonic day 13.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
7A
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Expression is detected in the ventral and dorsal spinal cord.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129/Ola * C57BL/6J
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord ventricular layer |
Weak |
Regionally restricted |
7D
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Expression is restricted to a few cells within or directly adjacent to the ventricular zone and is reduced compared to the wild type.
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Specimen
7G:
embryonic day 16.5
(more )
Specimen
7G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord |
Present |
Regionally restricted |
7G
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Expression is detected in the ventral and dorsal spinal cord.
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Specimen
7K:
(close )
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Genetic Background:
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involves: 129/Ola * C57BL/6J
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord |
Present |
Regionally restricted |
7K
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Expression is detected in the ventral and dorsal spinal cord but is reduced compared to wild type.
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Specimen
8A:
postnatal day 0
(more )
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8E:
(close )
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Genetic Background:
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involves: 129/Ola * C57BL/6J
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: spinal cord |
Present |
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8E
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Expression is reduced and delayed compared to wild type.
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Specimen
8I:
postnatal day 7
(more )
Specimen
8I:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8M:
(close )
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Genetic Background:
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involves: 129/Ola * C57BL/6J
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord |
Weak |
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8M
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Expression is greatly reduced and delayed compared to wild type.
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