Probe:	Nkx2.2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4B	Expression flanks Olig2 expression in the motorneuron precusor domain ventrally.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4F	Expression is detected in the ventral neuroepithelium of the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	5A Nkx2.2	Expression detected in the neuroepithelium and a few cells in the gray matter of the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	5B	Expression detected in the neuroepithelium and a few cells in the gray matter of the spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	5C Nkx2.2	Expression is detected in the ventricular zone and ventral gray matter of the spinal cord. Expression has also expanded dorsaly into the Olig2 positive domain.
TS21: spinal cord ventricular layer	Present	Regionally restricted	5C Nkx2.2	Expression is detected in the ventricular zone and ventral gray matter of the spinal cord. Expression has also expanded dorsaly into the Olig2 positive domain.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	5D	Expression is detected in the ventricular zone and ventral gray matter of the spinal cord. Expression has also expanded dorsaly into the Olig2 positive domain.
TS21: spinal cord ventricular layer	Present	Regionally restricted	5D	Expression is detected in the ventricular zone and ventral gray matter of the spinal cord. Expression has also expanded dorsaly into the Olig2 positive domain.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	5E Nkx2.2	Expression is detected in the neuroepithelium and the motorneuron precursor domain of the spinal cord.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	5F	Expression is detected in the neuroepithelium and the motorneuron precursor domain of the spinal cord.

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Present	Uniform	5G	Nkx2-2 and Olig2 expression domains overlap at this stage.

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Present	Uniform	5H	Nkx2-2 and Olig2 expression domains overlap at this stage.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	7C	Expression is detected in the ventral gray matter of the spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	7F	Expression is detected in the ventral gray matter of the spinal cord and is identical to the wild type.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted	7I	Expression is detected in the ventral gray matter of the spinal cord.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted	7M	Expression is detected in the ventral gray matter of the spinal cord and is identical to the wild type.

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Present	Regionally restricted	8C	Expression is detected in the white matter and the ventral gray matter of the spinal cord.

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Present	Regionally restricted	8G	Expression is detected in the white matter and the ventral gray matter of the spinal cord. Expression is gretaly reduced in the gray matter as compared to wild type.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present	Regionally restricted	8K

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Weak	Regionally restricted	8O	Expression is greatly reduced, especially in the white matter, in the entire spinal cord as compared to the wild type.