Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74236
Fu H, et al., Dual origin of spinal oligodendrocyte progenitors and evidence for the cooperative role of Olig2 and Nkx2.2 in the control of oligodendrocyte differentiation. Development. 2002 Feb;129(3):681-93
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2176469
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Gene symbol:
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Olig2
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Gene name:
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oligodendrocyte transcription factor 2
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Probe:
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Olig2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4A:
embryonic day 10.5
(more  )
Note: Double labeled: brown - Olig2; blue - Irx3 (assay MGI:2176522).
Specimen
4A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Olig2; blue - Irx3 (assay MGI:2176522).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
4A
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Expression is detected in the motorneuron precursor domain.
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Specimen
4B:
embryonic day 10.5
(more )
Note: Double labeled: blue - Olig2; brown - Nkx2-2 (assay MGI:2176510).
Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Olig2; brown - Nkx2-2 (assay MGI:2176510).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
4B
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Expression is detected in the motorneuron precursor domain.
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Specimen
4D:
embryonic day 12.5
(more )
Note: Double labeled: brown - Olig2; blue - Pdgfra (assay MGI:2176523).
Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Olig2; blue - Pdgfra (assay MGI:2176523).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4D
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Expression is detected in the ventral neuroepithelium.
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Specimen
4E:
embryonic day 12.5
(more )
Note: Double labeled: brown - Olig2; blue - Pdgfra (assay MGI:2176523).
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Olig2; blue - Pdgfra (assay MGI:2176523).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
4E
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Expression is detected in the ventral neuroepithelium.
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Specimen
5A:
embryonic day 12.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
5A Olig2
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Expression is detected in the neuroepithelium.
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Specimen
5B:
embryonic day 12.5
(more )
Note: Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
5B
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Expression detected in the neuroepithelium.
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Specimen
5C:
embryonic day 13.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
5C Olig2
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Expression is detected in the motorneuron precursor domain and the dorsal and ventral spinal cord.
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Specimen
5D:
embryonic day 13.5
(more )
Note: Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
5D
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Expression is detected in the motorneuron precursor domain and the dorsal and ventral spinal cord.
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Specimen
5E:
embryonic day 14.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Regionally restricted |
5E Olig2
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Expression is detected in the neuroepithelium of the whole spinal cord.
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Specimen
5F:
embryonic day 14.5
(more )
Note: Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Regionally restricted |
5F
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Expression is detected in the neuroepithelium of the whole spinal cord.
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Specimen
5G:
postnatal day 1
(more )
Note: Lateral region shown. Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Lateral region shown. Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: spinal cord |
Present |
Uniform |
5G
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Nkx2-2 and Olig2 expression domains overlap at this stage.
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Specimen
5H:
postnatal day 1
(more )
Note: Ventral region shown. Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
Specimen
5H:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Ventral region shown. Double labeled: brown - Olig2; blue - Nkx2-2 (assay MGI:2176510).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: spinal cord |
Present |
Uniform |
5H
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Nkx2-2 and Olig2 expression domains overlap at this stage.
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Specimen
7B:
embryonic day 13.5
(more )
Specimen
7B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
7B
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Expression is detected in the ventral and dorsal spinal cord.
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Specimen
7E:
(close )
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Genetic Background:
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involves: 129/Ola * C57BL/6J
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord ventricular layer |
Present |
Regionally restricted |
7E
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Expression is reduced compared to wild type and consists of a few cells within or adjacent to the ventricular zone.
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Specimen
7H:
embryonic day 16.5
(more )
Specimen
7H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord |
Present |
Regionally restricted |
7H
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Expression is detected in the ventral and dorsal spinal cord.
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Specimen
7L:
(close )
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Genetic Background:
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involves: 129/Ola * C57BL/6J
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord |
Weak |
Regionally restricted |
7L
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Expression is detected in the ventral and dorsal spinal cord but is reduced compared to wild type.
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Specimen
8B:
postnatal day 0
(more )
Specimen
8B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8F:
(close )
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Genetic Background:
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involves: 129/Ola * C57BL/6J
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: spinal cord |
Present |
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8F
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Expression is reduced and delayed as compared to wild type.
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Specimen
8J:
postnatal day 7
(more )
Specimen
8J:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8N:
(close )
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Genetic Background:
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involves: 129/Ola * C57BL/6J
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord |
Weak |
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8N
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Expression is greatly reduced and delayed as compared to wild type.
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