Probe:	Nrarp probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Strong	Regionally restricted	4A	Expression is in most of the central nervous system.
TS14: trunk paraxial mesenchyme	Strong	Regionally restricted	4A	There is an anterior stripe and a more extensive posterior domain.

Structure	Level	Pattern	Image	Note
TS15: central nervous system ganglion	Strong		4B
TS15: future forebrain	Strong		4B
TS15: future hindbrain	Strong		4B
TS15: future spinal cord	Strong		4B
TS15: future midbrain	Absent		4B
TS15: tail paraxial mesenchyme	Strong	Regionally restricted	4B	There is an anterior stripe and a more extensive posterior domain.
TS15: trunk paraxial mesenchyme	Strong	Regionally restricted	4B	There is an anterior stripe and a more extensive posterior domain.

Structure	Level	Pattern	Image	Note
TS15: central nervous system ganglion	Strong		4C
TS15: future forebrain	Strong		4C
TS15: future hindbrain	Strong		4C
TS15: future spinal cord	Strong		4C
TS15: future midbrain	Absent		4C

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Present		4D

Structure	Level	Pattern	Image	Note
TS17: tail paraxial mesenchyme	Present	Regionally restricted	4E	The expression consists of an anterior stripe and a more extensive posterior domain.
TS17: central nervous system	Strong		4E

Structure	Level	Pattern	Image	Note
TS17: tail paraxial mesenchyme	Present		4F
TS17: trunk paraxial mesenchyme	Present		4F

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Absent		4G
TS17: neural tube lateral wall	Present	Regionally restricted	4G	Expression is downregulated in motor neurons in the ventral horn.
TS17: neural tube roof plate	Present	Regionally restricted	4G	Expression is downregulated in motor neurons in the ventral horn.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Absent		4H
TS17: neural tube lateral wall	Present	Regionally restricted	4H	Expression is downregulated in motor neurons in the ventral horn.
TS17: neural tube roof plate	Present	Regionally restricted	4H	Expression is downregulated in motor neurons in the ventral horn.

Structure	Level	Pattern	Image	Note
TS17: anterior cardinal vein	Present		4I

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Present		5A
TS14: head paraxial mesenchyme	Present		5A
TS14: trunk paraxial mesenchyme	Present		5A

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present		5B
TS14: neural tube	Present		5B
TS14: future midbrain	Absent		5B
TS14: head paraxial mesenchyme	Absent		5B
TS14: prosencephalon	Absent		5B
TS14: trunk paraxial mesenchyme	Absent		5B

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present		5C
TS14: neural tube	Present		5C
TS14: future midbrain	Absent		5C
TS14: head paraxial mesenchyme	Absent		5C
TS14: prosencephalon	Absent		5C
TS14: trunk paraxial mesenchyme	Absent		5C

Structure	Level	Pattern	Image	Note
TS15: tail paraxial mesenchyme	Present	Regionally restricted	6A	There is an anterior stripe of expression and more extensive expression posteriorly.
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	6A	There is an anterior stripe of expression and more extensive expression posteriorly.

Structure	Level	Pattern	Image	Note
TS15: tail paraxial mesenchyme	Present	Regionally restricted	6B	There is an anterior stripe of expression and more extensive expression posteriorly. The anterior stripe coincides with the most caudal stripe of Uncx4.1 expression.
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	6B	There is an anterior stripe of expression and more extensive expression posteriorly. The anterior stripe coincides with the most caudal stripe of Uncx4.1 expression.

Structure	Level	Pattern	Image	Note
TS15: tail paraxial mesenchyme	Present	Regionally restricted	7A	Expression is in an anterior stripe and a more extensive posterior domain.
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	7A	Expression is in an anterior stripe and a more extensive posterior domain.

Structure	Level	Pattern	Image	Note
TS15: tail paraxial mesenchyme	Absent		7B
TS15: trunk paraxial mesenchyme	Absent		7B

Structure	Level	Pattern	Image	Note
TS15: tail paraxial mesenchyme	Present	Regionally restricted	7C	The anterior stripe of expression is missing in this mutant. The posterior expression domain is unaffected.
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	7C	The anterior stripe of expression is missing in this mutant. The posterior expression domain is unaffected.

Structure	Level	Pattern	Image	Note
TS15: tail paraxial mesenchyme	Present	Regionally restricted	7D	The anterior stripe of expression is diffuse and its domain is expanded. There is also posterior expression.
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	7D	The anterior stripe of expression is diffuse and its domain is expanded. There is also posterior expression.