Probe:	Raldh2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: foregut	Strong	Regionally restricted	1A	Strong expression along the anterior foregut, in the mesenchyme of the prospective trachea and lung primordia.
TS15: tracheal diverticulum	Strong	Regionally restricted	1A	Strong expression along the anterior foregut, in the mesenchyme of the prospective trachea and lung primordia.
TS15: lung	Strong	Regionally restricted	1A	Strong expression along the anterior foregut, in the mesenchyme of the prospective trachea and lung primordia.

Structure	Level	Pattern	Image	Note
TS15: tracheal diverticulum mesenchyme	Present	Regionally restricted	1B	High levels of expression in mesenchyme of prospective trachea, pleura and subpleural mesenchyme.
TS15: left lung rudiment mesenchyme	Present	Regionally restricted	1B	High levels of expression in mesenchyme of prospective trachea, pleura and subpleural mesenchyme.
TS15: right lung rudiment mesenchyme	Present	Regionally restricted	1B	High levels of expression in mesenchyme of prospective trachea, pleura and subpleural mesenchyme.
TS15: tracheal diverticulum epithelium	Absent		1B	All respiratory epithelia are negative.
TS15: left lung rudiment epithelium	Absent		1B	All respiratory epithelia are negative.
TS15: right lung rudiment epithelium	Absent		1B	All respiratory epithelia are negative.

Structure	Level	Pattern	Image	Note
TS15: left lung rudiment mesenchyme	Present	Regionally restricted	1C	High levels of expression in mesenchyme of prospective trachea, pleura and subpleural mesenchyme.
TS15: right lung rudiment mesenchyme	Present	Regionally restricted	1C	High levels of expression in mesenchyme of prospective trachea, pleura and subpleural mesenchyme.
TS15: tracheal diverticulum mesenchyme	Present	Regionally restricted	1C	High levels of expression in mesenchyme of prospective trachea, pleura and subpleural mesenchyme.
TS15: left lung rudiment epithelium	Absent		1C	All respiratory epithelia are negative.
TS15: right lung rudiment epithelium	Absent		1C	All respiratory epithelia are negative.
TS15: tracheal diverticulum epithelium	Absent		1C	All respiratory epithelia are negative.

Structure	Level	Pattern	Image	Note
TS16: respiratory system	Present	Graded	1D	Gradient of expression with highest levels proximally; signals decline towards the distal lung.
TS16: trachea mesenchyme	Strong		1D	Highest levels in tracheal mesenchyme and proximal lung mesenchyme (pleura).
TS16: left lung rudiment mesenchyme	Strong	Regionally restricted	1D	Highest levels in tracheal mesenchyme and proximal lung mesenchyme (pleura).
TS16: right lung rudiment mesenchyme	Strong	Regionally restricted	1D	Highest levels in tracheal mesenchyme and proximal lung mesenchyme (pleura).
TS16: pleura	Strong	Regionally restricted	1D	Highest levels in tracheal mesenchyme and proximal lung mesenchyme (pleura).
TS16: right lung rudiment	Present	Graded	1D	Signals decline towards the distal lung.

Structure	Level	Pattern	Image	Note
TS16: trachea mesenchyme	Strong		1E	Highest levels in tracheal mesenchyme and proximal lung mesenchyme (pleura).

Structure	Level	Pattern	Image	Note
TS19: lung	Present	Regionally restricted	1F	Concentration of signal at sites of less branching activity.

Structure	Level	Pattern	Image	Note
TS22: left lung	Present	Uniform	1H	Diffuse expression in lung.
TS22: right lung	Present	Uniform	1H	Diffuse expression in lung.

Structure	Level	Pattern	Image	Note
TS24: pleura	Present	Regionally restricted	1I	Expression in mesothelium at variable levels.

Structure	Level	Pattern	Image	Note
TS18: left lung rudiment	Strong	Regionally restricted	2A	Concentration of signal at sites of low branching activity; strong signals localize to basal regions of lobes.
TS18: right lung rudiment	Strong	Regionally restricted	2A	Concentration of signal at sites of low branching activity; strong signals localize to basal regions of lobes.

Structure	Level	Pattern	Image	Note
TS18: left lung rudiment	Strong	Regionally restricted	2C	Concentration of signal at sites of low branching activity; strong signals localize to basal regions of lobes.
TS18: right lung rudiment	Strong	Regionally restricted	2C	Concentration of signal at sites of low branching activity; strong signals localize to basal regions of lobes.

Structure	Level	Pattern	Image	Note
TS19: left lung rudiment	Present	Regionally restricted	2E	Expression along the medial and cranial surfaces of the left lung.

Structure	Level	Pattern	Image	Note
TS20: left lung	Present	Regionally restricted	2G	Distribution is uneven; highest levels cranially.
TS20: right lung	Present	Regionally restricted	2G	Distribution is uneven; highest levels cranially.