Gene Expression Data
Assay Details
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Assay
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Reference:
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J:62985
Helmbacher F, et al., Targeting of the EphA4 tyrosine kinase receptor affects dorsal/ventral pathfinding of limb motor axons. Development. 2000 Aug;127(15):3313-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2149752
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Gene symbol:
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Epha4
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Gene name:
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Eph receptor A4
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Probe:
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Epha4 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
embryonic day 11.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
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Expression along the entire length of the spinal cord.
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Specimen
5A:
embryonic day 11.5
(more )
Note: Embedded in albumin/gelatin.
Specimen
5A:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Embedded in albumin/gelatin.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Regionally restricted |
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From E12.5, expression is downregulated in medial motor column neurons; restricted to subpopulations of neurons in brachial and lumbar LMC.
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Specimen
8A:
embryonic day 13.5
(more )
Specimen
8A:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
8A
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Expression in brachial region (C7, C8) and lumbar region (L2-L5).
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Specimen
8B:
embryonic day 13.5
(more )
Specimen
8B:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
8B
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Expression in brachial region (C7, C8) and lumbar region (L2-L5).
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Specimen
8C:
embryonic day 14.5
(more )
Note: Embedded in albumin/gelatin.
Specimen
8C:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Embedded in albumin/gelatin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
8C
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At the L3-L5 levels. expression in most LMC neurons. At L4, expression in lateral (lLMC) and medial (mLMC) lateral motor columns.
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Specimen
8D:
embryonic day 14.5
(more )
Note: Embedded in albumin/gelatin.
Specimen
8D:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Embedded in albumin/gelatin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basal columns |
Present |
Regionally restricted |
8D
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At the L3-L5 levels. expression in most LMC neurons. At L4, expression in lateral (lLMC) and medial (mLMC) lateral motor columns.
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Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: basal columns |
Present |
Regionally restricted |
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At the L3-L5 levels. expression in most LMC neurons.
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Analysis Tools