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Gene Expression Data
Assay Details
Assay
Reference: J:70745 Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
Assay type: RNA in situ
MGI Accession ID: MGI:2148277
Gene symbol: Pax6
Gene name: paired box 6
Probe: Pax6 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2K: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon Strong Regionally restricted 2K Strong expression in ventral thalamus.
TS19: midbrain Strong Regionally restricted 2K Strong expression in posterior pretectum and tegmentum.
Specimen 2L: embryonic day 11.5; Emx2tm1Sia/Emx2tm1Sia, Otx2tm1Sia/Otx2+ (more )
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Structure Level Pattern Image Note
TS19: midbrain Strong Regionally restricted 2L Strong expression in basal posterior pretectum and midbrain tegmentum.
Specimen 3E: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: telencephalon Present 3E
TS15: diencephalon Present 3E
Specimen 3F: embryonic day 9.5; Emx2tm1Sia/Emx2tm1Sia, Otx2tm1Sia/Otx2+ (more )
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Structure Level Pattern Image Note
TS15: telencephalon Absent 3F
TS15: diencephalon Absent 3F
TS15: eye Present 3F
Specimen 4C: embryonic day 8.0 (more )
Note: 6-somite stage.
Structure Level Pattern Image Note
TS12: future hindbrain Present 4C
TS12: future prosencephalon Present 4C
TS12: future spinal cord Present 4C
TS12: eye Present Regionally restricted 4C Expression in the laterocaudal component of the incipient optic cup.
Specimen 4D: embryonic day 8.0; Emx2tm1Sia/Emx2tm1Sia, Otx2tm1Sia/Otx2+ (more )
Note: 6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Structure Level Pattern Image Note
TS12: eye Present 4D
TS12: future hindbrain Present 4D
TS12: future spinal cord Present 4D
TS12: future prosencephalon Absent 4D Authors state that expression was scarcely observed in the forebrain of double mutants.
Specimen not shown: embryonic day 8.0; Emx2tm1Sia/Emx2tm1Sia, Otx2tm1Sia/Otx2+ (more )
Note: 4-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Structure Level Pattern Image Note
TS12: future brain Absent Authors state that Pax6 expression is not detected in double mutants at the 4-somite stage.
Specimen 7M: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forebrain Present 7M
Specimen 7O: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 7O Expression in ventral thalamus.
TS20: midbrain Present Regionally restricted 7O Expression in posterior pretectum.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory