Gene Expression Data
Assay Details
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Assay
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Reference:
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J:69852
Colvin JS, et al., Lung hypoplasia and neonatal death in Fgf9-null mice identify this gene as an essential regulator of lung mesenchyme. Development. 2001 Jun;128(11):2095-106
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2137010
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Gene symbol:
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Fgf10
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Gene name:
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fibroblast growth factor 10
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Probe:
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Fgf10 probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
C,E:
embryonic day 12.5
(more  )
Specimen
C,E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
D,F -/-:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Fgf9tm1Dor/Fgf9tm1Dor
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: left lung mesenchyme |
Present |
Regionally restricted |
5D
,
5F
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Expression is in mesenchyme distal to budding airways. Expression in this mutant is the same as in wild-type embryos.
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| TS20: right lung mesenchyme |
Present |
Regionally restricted |
5D
,
5F
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Expression is in mesenchyme distal to budding airways. Expression in this mutant is the same as in wild-type embryos.
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Specimen
G,I:
embryonic day 14.5
(more )
Specimen
G,I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: left lung mesenchyme |
Present |
Regionally restricted |
5I
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Foci of expression were seen in most distal lung buds.
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| TS22: right lung mesenchyme |
Present |
Regionally restricted |
5I
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Foci of expression were seen in most distal lung buds.
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| TS22: lung |
Moderate |
Regionally restricted |
5G
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Expression is in mesenchyme between distal lung buds.
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Specimen
H,J -/-:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Fgf9tm1Dor/Fgf9tm1Dor
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: left lung mesenchyme |
Present |
Regionally restricted |
5J
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In mutant animals, interbud mesenchyme often lacks expression.
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| TS22: right lung mesenchyme |
Present |
Regionally restricted |
5J
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In mutant animals, interbud mesenchyme often lacks expression.
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| TS22: lung |
Weak |
Regionally restricted |
5H
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Expression is in mesenchyme between lung buds.
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Specimen
Not Shown:
embryonic day 13.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not Shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Fgf9tm1Dor/Fgf9tm1Dor
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools