Gene Expression Data
Assay Details
Assay
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Reference:
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J:48569
Grove EA, et al., The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development. 1998 Jun;125(12):2315-25
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2136857
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Gene symbol:
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Wnt5a
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Gene name:
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wingless-type MMTV integration site family, member 5A
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Probe:
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Wnt5a probe1
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Probe preparation:
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Antisense RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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One- and two-color in situ hybridization was performed using digoxygenin- and/or fluorescein-labeled probes, detected with alkaline phosphatase-conjugated anti-digoxigenin or anti-fluorescein antibodies. Color reactions were carried out using different alkaline phosphatase substrates, resulting in different colored products as indicated in some of the figure legends.
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Results
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Specimen
4D:
embryonic day 11.5
(more )
Specimen
4D:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
4D
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The cortical hem (see below) is delineated by the expression of Wnt2b, Wnt3a and Wnt5a.
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Specimen
3H:
embryonic day 12.5
(more )
Specimen
3H:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Strong |
Regionally restricted |
3H
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Curved band of strong expression along medial face of telencephalon; marks the cortical hem.
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TS20: head mesenchyme |
Present |
Regionally restricted |
3H
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Expression in head mesenchyme invading the choroid plexus epithelium to form the stroma.
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TS20: choroid invagination |
Absent |
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3H
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No expression in the choroid plexus epithelium.
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Specimen
3I:
embryonic day 12.5
(more )
Specimen
3I:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: head mesenchyme |
Present |
Regionally restricted |
3I
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Expression in mesenchymal cells that are invading the medial wall of the telencephalon to form the stromal layer of the choroid plexus.
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Specimen
7B:
(close )
Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Weak |
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7B
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In brains of non-exencephalic homozygous mutant mice, telencephalic expression severely downregulated (weak and diffuse). There is no expression in tissue that might correspond to the cortical hem.
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Specimen
not shown:
(close )
Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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Specimen
5C:
embryonic day 13.5
(more )
Specimen
5C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: cortical plate |
Present |
Regionally restricted |
5C
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Expression in the cortical plate of the entire medial cerebral cortex, including the hippocampus (postmitotic neurons in the pyramidal cell layer) and adjacent limbic cortical areas. Expression marks the shrinking cortical hem.
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TS21: telencephalon |
Present |
Regionally restricted |
5C
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Expression in the cortical plate of the entire medial cerebral cortex, including the hippocampus (postmitotic neurons in the pyramidal cell layer) and adjacent limbic cortical areas. Expression marks the shrinking cortical hem.
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TS21: head mesenchyme |
Present |
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5C
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Expression also labels head mesenchymal cells.
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Specimen
5D:
embryonic day 16.5
(more )
Specimen
5D:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: telencephalon |
Present |
Regionally restricted |
5D
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Overlapping expression of Wnt2b, Wnt3a and Wnt5a marks a few cells along the lateral margin of the hippocampal fimbria-fornix. Expression largely confined to the hippocampal dentate gyrus.
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Specimen
not shown:
(close )
Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS24: telencephalon |
Absent |
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Five non-exencephalic homozygous mutants showed no expression in the medial telencephalon.
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