Gene Expression Data
Assay Details
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Assay
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Reference:
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J:2140
Bally-Cuif L, et al., Relationship between Wnt-1 and En-2 expression domains during early development of normal and ectopic met-mesencephalon. Development. 1992 Aug;115(4):999-1009
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1935118
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Gene symbol:
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En2
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Gene name:
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engrailed 2
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Probe:
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En2 cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
E7.5:
embryonic day 7.5
(more  )
Specimen
E7.5:
(close  )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Absent |
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Authors state that expression is never detected at E7.5.
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Specimen
E8.5:
embryonic day 8.5
(more )
Specimen
E8.5:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Weak |
Regionally restricted |
1A En-2
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Some embryos show expression, generally weak, in a region apparently overlapping the wnt1-expressing domain. Most embryos show no expression.
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Specimen
E9.5:
embryonic day 9.5
(more )
Specimen
E9.5:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Graded |
1B En-2
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Expression encirclin neural tube in met-mesencephalic region, corresponding to presumptive cerebellum, isthmus and colliculi; highest expression at constriction and rostrally; decreases rostrally and caudally.
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| TS15: future midbrain |
Present |
Graded |
1B En-2
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Expression encirclin neural tube in met-mesencephalic region, corresponding to presumptive cerebellum, isthmus and colliculi; highest expression at constriction and rostrally; decreases rostrally and caudally.
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Specimen
E10.5:
embryonic day 10.5
(more )
Specimen
E10.5:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Graded |
2A En-2
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Expression encirclin neural tube in met-mesencephalic region, corresponding to presumptive cerebellum, isthmus and colliculi; highest expression at constriction and rostrally; decreases rostrally and caudally.
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| TS17: midbrain |
Present |
Graded |
2A En-2
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Expression encirclin neural tube in met-mesencephalic region, corresponding to presumptive cerebellum, isthmus and colliculi; highest expression at constriction and rostrally; decreases rostrally and caudally.
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Specimen
E11.5:
embryonic day 11.5
(more )
Specimen
E11.5:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 11.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Graded |
2B En-2
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Expression encirclin neural tube in met-mesencephalic region, corresponding to presumptive cerebellum, isthmus and colliculi; highest expression at constriction and rostrally; decreases rostrally and caudally.
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| TS20: midbrain |
Present |
Graded |
2B En-2
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Expression encirclin neural tube in met-mesencephalic region, corresponding to presumptive cerebellum, isthmus and colliculi; highest expression at constriction and rostrally; decreases rostrally and caudally.
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Specimen
E12.5:
embryonic day 12.5
(more )
Specimen
E12.5:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
Regionally restricted |
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Authors report "maximal En2 expression in the Wnt1-positive area."
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Specimen
E13.5:
embryonic day 13.5
(more )
Specimen
E13.5:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brain |
Present |
Regionally restricted |
2C En-2
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Authors report that En2 expression becomes more complex; in a more restricted domain of the germinal zone, plus some post-mitotic nuclei, notably in the isthmus and cerebellum.
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Analysis Tools