Antibody:	anti-NCAM polyclonal
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	1A	Staining in the neuroepithelium of the closing neural tube in the posterior region.
TS14: trunk mesenchyme	Present	Regionally restricted	1A	Staining in the apical area of the mesoderm.
TS14: notochord	Weak		1A
TS14: gut	Absent		1A
TS14: surface ectoderm	Absent		1A

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	1B	In a region were the neural folds begin to fuse staining is most intense in the lateral portions of the neural tube.
TS14: trunk mesenchyme	Present	Regionally restricted	1B	Less staining in the mesoderm surrounding the neural tube.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	1C	Staining is most intense in the lateral portions of the neural tube in a region where the neural folds appose one another just prior to fusion.
TS14: trunk mesenchyme	Present	Regionally restricted	1C	Staining is in the mesoderm surrounding the neural tube.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural plate	Present	Regionally restricted	1D	Staining in the open caudal region; even distribution throughout the neuroepithelial tissue.
TS14: neural tube	Present	Regionally restricted	1D	Staining in the open caudal region; even distribution throughout the neuroepithelial tissue.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	2B	Section through the hindbrain region showing the foregut. Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain region.
TS13: future prosencephalon	Present	Regionally restricted	2B	Section through the hindbrain region showing the foregut. Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain region.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		2C	Open caudal region of the embryo. Staining in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	2D	Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain regions.
TS13: future prosencephalon	Present	Regionally restricted	2D	Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain regions.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	2E	Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain regions.
TS13: future prosencephalon	Present	Regionally restricted	2E	Staining in the lateral portions of the closing neural tube in the forebrain and hindbrain regions.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	2F	Open caudal region of the embryo. Expression in the lateral portions of the neural tube.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Strong		3A	Strong staining along the lateral portions of the neural tube. Sections through the trunk, open neural tube, and caudal regions.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Strong		3B	Strong staining along the lateral portions of the neural tube. Sections through the trunk, open neural tube, and caudal regions.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Strong		3C	Strong staining along the lateral portions of the neural tube. Sections through the trunk, open neural tube, and caudal regions.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong			Strong staining along the lateral portions of the neural tube.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present		3D	Staining is reduced compared to Sp-/- embryos. Sections through the trunk, open neural tube, and caudal regions.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present		3E	Staining is reduced compared to Sp-/- embryos. Sections through the trunk, open neural tube, and caudal regions.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present		3F	Staining is reduced compared to Sp-/- embryos. Sections through the trunk, open neural tube, and caudal regions.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present			Staining is reduced compared to Sp-/- embryos.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present			Staining is similar to wild-type but the heterozygotes showed more variability.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present			Less dramatic differences between mutant and non-mutant embryos in staining patterns.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present			Less dramatic differences between mutant and non-mutant embryos in staining patterns.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present			Less dramatic differences between mutant and non-mutant embryos in staining patterns.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present			Less dramatic differences between mutant and non-mutant embryos in staining patterns.