Hoxb3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:68216 Akasaka T, et al., Mice doubly deficient for the Polycomb Group genes Mel18 and Bmi1 reveal synergy and requirement for maintenance but not initiation of Hox gene expression. Development. 2001 May;128(9):1587-97
Assay type:	RNA in situ
MGI Accession ID:	MGI:1933566
Gene symbol:	Hoxb3
Gene name:	homeobox B3

Probe:	Hoxb3 probe6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 1C Mel18+/+; Bmi1+/+: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme derived from neural crest	Absent		1C Mel18+/+; Bmi1+/+ lower , 1C Mel18+/+; Bmi1+/+ upper	Absent in neural-crest derived cells anterior to the otic vesicle.

Specimen 1C Mel18-/-; Bmi1+/+: embryonic day 9.5; Pcgf2^tm1Hko/Pcgf2^tm1Hko (more

)

Structure	Level	Image	Note
TS15: head mesenchyme derived from neural crest	Absent	1C Mel18-/-; Bmi1+/+ lower , 1C Mel18-/-; Bmi1+/+ upper	Absent in neural-crest derived cells anterior to the otic vesicle.
TS15: rhombomere 4	Present	1C Mel18-/-; Bmi1+/+ lower	A slight de-repression was seen.
TS15: neural tube	Present	1C Mel18-/-; Bmi1+/+ lower , 1C Mel18-/-; Bmi1+/+ upper

Specimen 1C Mel18+/+; Bmi1-/-: embryonic day 9.5; Bmi1^tm1Brn/Bmi1^tm1Brn (more

)

Structure	Level	Image	Note
TS15: head mesenchyme derived from neural crest	Absent	1C Mel18+/+; Bmi1-/- upper , 1C Mel18+/+; Bmi1-/- lower	Absent in neural-crest derived cells anterior to the otic vesicle.
TS15: rhombomere 4	Present	1C Mel18+/+; Bmi1-/- lower	A slight de-repression was seen.
TS15: neural tube	Present	1C Mel18+/+; Bmi1-/- lower , 1C Mel18+/+; Bmi1-/- upper

Specimen 1C Mel18-/-; Bmi1+/-: embryonic day 9.5; Pcgf2^tm1Hko/Pcgf2^tm1Hko, Bmi1^tm1Brn/Bmi1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	1C Mel18-/-; Bmi1+/- upper	Expression was seen in neural crest-derived cells migrating into the second branchial arches. (observed in 2 out of 3 embryos)
TS15: head mesenchyme derived from neural crest	Present		1C Mel18-/-; Bmi1+/- upper , 1C Mel18-/-; Bmi1+/- lower
TS15: migrating neural crest	Present		1C Mel18-/-; Bmi1+/- upper , 1C Mel18-/-; Bmi1+/- lower
TS15: rhombomere 4	Present		1C Mel18-/-; Bmi1+/- lower	A slight de-repression was seen.
TS15: neural tube	Present		1C Mel18-/-; Bmi1+/- lower , 1C Mel18-/-; Bmi1+/- upper

Specimen 1C Mel18+/-; Bmi1-/-: embryonic day 9.5; Pcgf2^tm1Hko/Pcgf2⁺, Bmi1^tm1Brn/Bmi1^tm1Brn (more

)

Structure	Level	Image	Note
TS15: head mesenchyme	Present	1C Mel18+/-; Bmi1-/- lower , 1C Mel18+/-; Bmi1-/- upper	Expression in the mesenchymal cells beneath the optic vesicle. (seen in 2 out of 4 embryos)
TS15: 1st branchial arch	Present	1C Mel18+/-; Bmi1-/- upper	(seen in 2 out of 4 embryos)
TS15: 2nd branchial arch	Present	1C Mel18+/-; Bmi1-/- upper	(seen in 2 out of 4 embryos)
TS15: rhombomere 4	Strong	1C Mel18+/-; Bmi1-/- lower
TS15: neural tube	Present	1C Mel18+/-; Bmi1-/- upper , 1C Mel18+/-; Bmi1-/- lower

3A ©

Specimen 3A: embryonic day 9.5; Pcgf2^tm1Hko/Pcgf2^tm1Hko, Bmi1^tm1Brn/Bmi1^tm1Brn (more

)

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		3A	The anterior boundary of expression was shifted up to the forebrain.

3B ©

Specimen 3B: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3B	Expression was restricted to the presumptive cervical region of the neural tube.

Specimen not shown: embryonic day 9.5; Pcgf2^tm1Hko/Pcgf2^tm1Hko (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted		Expression was restricted to the presumptive cervical region of the neural tube.

Specimen not shown: embryonic day 9.5; Bmi1^tm1Brn/Bmi1^tm1Brn (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted		Expression was restricted to the presumptive cervical region of the neural tube.

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