Gene Expression Data
Assay Details
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Assay
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Reference:
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J:21617
Gunther T, et al., Open brain, a new mouse mutant with severe neural tube defects, shows altered gene expression patterns in the developing spinal cord. Development. 1994 Nov;120(11):3119-30
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933417
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Probe:
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Pax6 probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
5A, 5C:
embryonic day 12.5
(more  )
Specimen
5A, 5C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
5A
,
5C
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Expression in the basal plate of the ventricular layer near the sulcus limitans, and low level expression in the ependymal layer of the alar plate.
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| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
5A
,
5C
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Expression in the basal plate of the ventricular layer near the sulcus limitans, and low level expression in the ependymal layer of the alar plate.
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Specimen
5B, 5D:
embryonic day 12.5; Rab23opb/ Rab23opb
(more )
Note: The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
Specimen
5B, 5D:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
5D
,
5B
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Expression was not detected in the dorsalmost region. In other regions of the presumptive alar plate, expression was significantly stronger than in wild type embryos. The ventralmost region negative for expression appeared to be enlarged.
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| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
5D
,
5B
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Expression was not detected in the dorsalmost region. In other regions of the presumptive alar plate, expression was significantly stronger than in wild type embryos. The ventralmost region negative for expression appeared to be enlarged.
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Specimen
5E:
embryonic day 12.5; Rab23opb/ Rab23opb
(more )
Note: The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
Specimen
5E:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
5E
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In less severely affected spinal cord regions, expression extended to the dorsalmost cells; an elevated level of expression could be observed in the dorsal half.
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| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
5E
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In less severely affected spinal cord regions, expression extended to the dorsalmost cells; an elevated level of expression could be observed in the dorsal half.
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Analysis Tools
