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Gene Expression Data
Assay Details
Assay
Reference: J:65599 Gaufo GO, et al., Hoxb1 controls effectors of sonic hedgehog and mash1 signaling pathways. Development. 2000 Dec;127(24):5343-54
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1928136
Gene symbol: Phox2b
Gene name: paired-like homeobox 2b
Antibody: anti Phox2b
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 6a: embryonic day 9.5 (more )
Note: Double labeled: green - Phox2b; red - TUNEL.
Structure Level Pattern Image Note
TS15: rhombomere 4 Present Regionally restricted 6a Expression is in the ventral neural tube throughout the neural epithelium from the ventricular layer to the outer marginal layer.
Specimen 6b: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Double labeled: green - Phox2b; red - TUNEL.
Structure Level Pattern Image Note
TS15: rhombomere 4 Present Regionally restricted 6b In this mutant the number of expressing cells is greatly reduced. The most ventral expression is maintained.
Specimen 6c: embryonic day 10.0 (more )
Note: Double labeled: green - Phox2b; red - TUNEL.
Structure Level Pattern Image Note
TS16: rhombomere 4 Present Regionally restricted 6c Expression is in the ventral neural tube throughout the neural epithelium from the ventricular layer to the outer marginal layer.
Specimen 6d: embryonic day 10.0; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Double labeled: green - Phox2b; red - TUNEL.
Structure Level Pattern Image Note
TS16: rhombomere 4 ventricular layer Present Single cells 6d
Specimen 6e: embryonic day 11.0 (more )
Note: Double labeled: green - Phox2b; red - Isl1 (assay MGI:1928095).
Structure Level Pattern Image Note
TS18: rhombomere 4 ventricular layer Present Regionally restricted 6e Expression is in distinct dorsal and ventral regions. Expression is in the ventral progenitor domain.
Specimen 6f: embryonic day 11.0; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Double labeled: green - Phox2b; red - Isl1 (assay MGI:1928095).
Structure Level Pattern Image Note
TS18: rhombomere 4 marginal layer Present 6f
TS18: rhombomere 4 ventricular layer Absent 6f There is no expression in the ventral progenitor domain.
Specimen 6g: embryonic day 11.5 (more )
Note: Double labeled: green - Phox2b; red - Nkx2-2 (assay MGI:1928093). Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Structure Level Pattern Image Note
TS18: rhombomere 4 Present Regionally restricted 6g Expression is in the ventral progenitor domain with a few postmitotic cells expresing.
Specimen 6h: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Double labeled: green - Phox2b; red - Nkx2-2 (assay MGI:1928093). Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Structure Level Pattern Image Note
TS18: rhombomere 4 Present Regionally restricted 6h In this mutant the is no expression in the ventral progenitor domain.
TS18: rhombomere 4 marginal layer Present Regionally restricted 6h In this mutant the ventral expression region is expanded.
TS18: rhombomere 4 ventricular layer Present Regionally restricted 6h In this mutant the ventral expression region is expanded.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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