Gene Expression Data
Assay Details
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Assay
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Reference:
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J:65599
Gaufo GO, et al., Hoxb1 controls effectors of sonic hedgehog and mash1 signaling pathways. Development. 2000 Dec;127(24):5343-54
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1928095
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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Anti-Isl1
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Detection system:
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Secondary antibody coupled to Texas Red
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Results
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Specimen
4e:
embryonic day 11.5
(more  )
Note: Double labeled: red - Isl1; green - Hoxb1 (assay MGI:1928087). Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Specimen
4e:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Isl1; green - Hoxb1 (assay MGI:1928087). Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 4 |
Present |
Regionally restricted |
4e
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Expression is in the facial branchiomotor neurons, the contralateral vestibuloacoustic efferent neurons, and a population of ventrolateral neurons.
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Specimen
4f:
embryonic day 11.5; Hoxb1tm5Mrc/ Hoxb1tm5Mrc
(more )
Note: Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Specimen
4f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1tm5Mrc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 4 |
Present |
Regionally restricted |
4f
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In this mutant the facial branchiomotor neurons and contralateral vestibuloacoustic efferent neurons are missing. Expression in the ventrolateral neurons has expanded dorsolaterally due to population expansion.
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Specimen
6e:
embryonic day 11.0
(more )
Note: Double labeled: red - Isl1; green - Phox2b (assay MGI:1928136).
Specimen
6e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Isl1; green - Phox2b (assay MGI:1928136).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 4 |
Present |
Regionally restricted |
6e
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Expression is in the facial branchiomotor neurons, the contralateral vestibuloacoustic efferent neurons, and a population of ventrolateral neurons.
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Specimen
6f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1tm5Mrc
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Isl1; green - Phox2b (assay MGI:1928136).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 4 |
Present |
Regionally restricted |
6f
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In this mutant the facial branchiomotor neurons and contralateral vestibuloacoustic efferent neurons are missing. Expression in the ventrolateral neurons has expanded dorsolaterally due to population expansion.
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