Fgf4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:60091 Moon AM, et al., Normal limb development in conditional mutants of Fgf4. Development. 2000 Mar;127(5):989-96
Assay type:	RNA in situ
MGI Accession ID:	MGI:1353164
Gene symbol:	Fgf4
Gene name:	fibroblast growth factor 4

Probe:	Fgf4 probe11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

3A F4^+/+ ©

Specimen 3A F4+/+: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		3A F4^+/+
TS17: hindlimb bud apical ectodermal ridge	Present		3A F4^+/+

3A F4^AP/Null ©

Specimen 3A F4^AP/Null: embryonic day 10.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		3A F4^AP/Null	Expression in this mutant is the same as in wild-type embryos.
TS17: hindlimb bud apical ectodermal ridge	Present		3A F4^AP/Null	Expression in this mutant is the same as in wild-type embryos.

3A F4^AP/Null;T ©

Specimen 3A F4^AP/N;T: embryonic day 10.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc, Tg(Rarb-cre)1Mrc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Absent		3A F4^AP/Null;T
TS17: hindlimb bud apical ectodermal ridge	Strong		3A F4^AP/Null;T

3B F4^+/+ ©

Specimen 3B F4+/+: embryonic day 10.0 (more

)
Note: 30 somites.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud apical ectodermal ridge	Present		3B F4^+/+

3B F4^AP/Null ©

Specimen 3B F4^AP/Null: embryonic day 10.0; Fgf4^tm1Mrc/Fgf4^tm2Mrc (more

)
Note: 30 somites.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud apical ectodermal ridge	Present		3B F4^AP/Null	Expression in this mutant is the same as in wild-type embryos.

3B F4^AP/Null;T ©

Specimen 3B F4^AP/N;T: embryonic day 10.0; Fgf4^tm1Mrc/Fgf4^tm2Mrc, Tg(Rarb-cre)1Mrc/0 (more

)
Note: Conditional mutant. 30 somites.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud apical ectodermal ridge	Absent		3B F4^AP/Null;T

3C F4^+/+ ©

Specimen 3C F4+/+: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		3C F4^+/+

3C F4^AP/Null ©

Specimen 3C F4^AP/Null: embryonic day 10.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		3C F4^AP/Null	Expression in this mutant is the same as in wild-type embryos.

Specimen 3C F4^AP/N;T: embryonic day 10.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc, Tg(Rarb-cre)1Mrc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Absent		3C F4^AP/Null;T

3D F4^+/+ ©

Specimen 3D F4+/+: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		3D F4^+/+

Specimen 3D F4^AP/Null: embryonic day 11.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc (more

)

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		3D F4^AP/Null	Expression in this mutant is the same as in wild-type embryos.

Specimen 3D F4^AP/N;T: embryonic day 11.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc, Tg(Rarb-cre)1Mrc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Absent		3D F4^AP/Null;T

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