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Gene Expression Data
Assay Details
Assay
Reference: J:41540 Rossel M, et al., Mice mutant for both Hoxa1 and Hoxb1 show extensive remodeling of the hindbrain and defects in craniofacial development. Development. 1999 Nov;126(22):5027-40
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1346699
Gene symbol: Egr2
Gene name: early growth response 2
Antibody: rabbit Krox-20 antibody
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 4E: embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1+, Hoxb1tm3Mrc/Hoxb1+ (more )
Note: Double labeled: red/brown - Egr2; purple - Mafb (assay MGI:1346698).
Structure Level Pattern Image Note
TS13: rhombomere 3 Present 4E
Specimen 4F: embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1tm1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Note: Double labeled: red/brown - Egr2; purple - Mafb (assay MGI:1346698). (G) Higher magnification of the embryo shown in (F).
Structure Level Pattern Image Note
TS13: future hindbrain Present Regionally restricted 4G , 4F Expression extends further caudally in this mutant than in controls.
Specimen 7A: embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1+, Hoxb1tm3Mrc/Hoxb1+ (more )
Note: Double labeled: green - Egr2; red - Hoxb1 (assay MGI:1346705).
Structure Level Pattern Image Note
TS13: rhombomere 3 Strong 7A
TS13: rhombomere 5 Weak 7A
Specimen 7B: embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1tm1Mrc (more )
Note: Double labeled: green - Egr2; red - Hoxb1 (assay MGI:1346705).
Structure Level Pattern Image Note
TS13: rhombomere 3 Present 7B The r3 expression domain is larger and extends more posteriorly than in control embryos.
TS13: rhombomere 5 Present 7B
Specimen 7C: embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1tm1Mrc, Hoxb1tm3Mrc/Hoxb1+ (more )
Note: Double labeled: green - Egr2; red - Hoxb1 (assay MGI:1346705).
Structure Level Pattern Image Note
TS13: future hindbrain Present 7C The caudal boundary of expression is extended even more posteriorly than in aaBb embryos and control embryos.
Specimen 7D: embryonic day 10.0; Hoxa1tm1Mrc/Hoxa1+, Hoxb1tm3Mrc/Hoxb1+ (more )
Note: Double labeled: green - Egr2; red - Hoxb1 (assay MGI:1346705).
Structure Level Pattern Image Note
TS16: neural tube Absent 7D
Specimen 7E: embryonic day 10.0; Hoxa1tm1Mrc/Hoxa1tm1Mrc, Hoxb1tm3Mrc/Hoxb1+ (more )
Note: Double labeled: green - Egr2; red - Hoxb1 (assay MGI:1346705).
Structure Level Pattern Image Note
TS16: neural tube Absent 7E
Specimen 8A: embryonic day 9.0; Hoxa1tm1Mrc/Hoxa1+, Hoxb1tm3Mrc/Hoxb1+ (more )
Note: 10-11 somites.
Structure Level Pattern Image Note
TS14: rhombomere 3 Present 8A
TS14: rhombomere 5 Present 8A
Specimen 8B: embryonic day 9.0; Hoxa1tm1Mrc/Hoxa1tm1Mrc (more )
Note: 10-11 somites.
Structure Level Pattern Image Note
TS14: rhombomere 3 Present 8B Expression is limited to a single band (r3) that is broader and extends to a more posterior level than in control embryos.
Specimen 8C: embryonic day 9.0; Hoxa1tm1Mrc/Hoxa1tm1Mrc, Hoxb1tm3Mrc/Hoxb1tm3Mrc (more )
Note: 10-11 somites.
Structure Level Pattern Image Note
TS14: future hindbrain Present 8C , 8D Expression is limited to a single band that is broader and extends to a more posterior level than in control embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory