Gene Expression Data
Assay Details
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Assay
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Reference:
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J:16332
Koseki H, et al., A role for Pax-1 as a mediator of notochordal signals during the dorsoventral specification of vertebrae. Development. 1993 Nov;119(3):649-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1343601
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Antibody:
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anti-TN1-123
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Detection system:
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horseradish peroxidase
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Assay notes:
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The reference for the antibody describes the production of two T antisera. It is possible that an antibody to the carboxyl terminus was used in this assay (MGI:1335398).
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Results
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Specimen
6.A:
embryonic day 9.5
(more  )
Note: 24-somite stage.
Specimen
6.A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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24-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
Uniform |
6A
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Staining was homogeneous in the notochord from infundibulum to the tailbud.
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Specimen
6.B:
embryonic day 9.5; Etn2Sd/ Etn2Sd
(more )
Note: 21-somite stage.
Specimen
6.B:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6ByJ
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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21-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
Regionally restricted |
6B
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Staining was heterogeneous in the head and cervical region and also discontinuous in the cervical region.
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Specimen
6.C:
embryonic day 10.5
(more )
Note: 37-somite stage.
Specimen
6.C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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37-somite stage.
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Specimen
6.D:
embryonic day 10.5; Etn2Sd/ Etn2Sd
(more )
Note: 35-somite stage.
Specimen
6.D:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6ByJ
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: notochord |
Present |
Regionally restricted |
6D
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Only rudimentary staining was occasionally seen in the cervicothoracic region; no epxression was detected in the caudal region.
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Specimen
6.E:
embryonic day 10.5
(more )
Note: 37-somite stage.
Specimen
6.E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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37-somite stage.
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Specimen
6.F:
embryonic day 10.5; Etn2Sd/ Etn2Sd
(more )
Note: 35-somite stage.
Specimen
6.F:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6ByJ
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: notochord |
Present |
Regionally restricted |
6F
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Only rudimentary staining was occasionally seen in the cervicothoracic region; no epxression was detected in the caudal region.
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