Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45709
Melloy PG, et al., No turning, a mouse mutation causing left-right and axial patterning defects. Dev Biol. 1998 Jan 1;193(1):77-89
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1343319
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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nodal probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 8.0
(more  )
Note: 3-5 somites.
Specimen
5A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
5A
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Nodal is expressed on the left side of normal embryos.
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Specimen
5B:
embryonic day 8.0; nt/ nt
(more )
Note: 3-5 somites.
Specimen
5B:
(close )
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Genetic Background:
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CD-1
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Mutant Allele(s):
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nt/nt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
5B
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In nt embryos, nodal expression is variable, as individuals may show expression on the right side, left side, or on both sides. Bilateral expression of nodal is shown in Figure 5.
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Analysis Tools