Probe:	Hox-4.3 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	6A -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: metanephros	Present	Regionally restricted	6A -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: nervous system	Present	Regionally restricted	6A -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: intestine mesenchyme	Present	Regionally restricted	6A -4.3	Expression in gut mesenchyme is restricted to posterior regions of the intestine.
TS20: central nervous system	Present	Regionally restricted	6A -4.3	Transcripts extend far more anteriorly in the CNS, since the boundary of expression lies in the very posterior hindbrain, close to its junction with the spinal cord.
TS20: hindbrain	Present	Regionally restricted	6A -4.3	Transcripts extend far more anteriorly in the CNS, since the boundary of expression lies in the very posterior hindbrain, close to its junction with the spinal cord.
TS20: spinal cord	Present	Regionally restricted	6A -4.3	Transcripts extend far more anteriorly in the CNS, since the boundary of expression lies in the very posterior hindbrain, close to its junction with the spinal cord.
TS20: dorsal root ganglion	Present	Regionally restricted	6A -4.3	Expression extends very anteriorly in the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS20: esophagus	Absent
TS20: lung	Absent
TS20: stomach	Absent

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	6C -4.3	Transcripts extend far more anteriorly in the CNS, since the boundary of expression lies in the very posterior hindbrain, close to its junction with the spinal cord.
TS20: hindbrain	Present	Regionally restricted	6C -4.3	Transcripts extend far more anteriorly in the CNS, since the boundary of expression lies in the very posterior hindbrain, close to its junction with the spinal cord.
TS20: spinal cord	Present	Regionally restricted	6C -4.3	Transcripts extend far more anteriorly in the CNS, since the boundary of expression lies in the very posterior hindbrain, close to its junction with the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	7C -4.3	Labelling by the Hox-4.3 probe is restricted to several central regions of the limbs (precartilaginous condensations) in a way similar to that of Hox-4.4.
TS20: hindlimb	Present	Regionally restricted	7C -4.3	Labelling by the Hox-4.3 probe is restricted to several central regions of the limbs (precartilaginous condensations) in a way similar to that of Hox-4.4.
TS20: body wall mesenchyme	Present	Regionally restricted	7C -4.3	Strong Hox-4.3 labelling is seen in superficial mesenchyme at the base of the head, up to very lateral regions. This labelling is similar to that obtained with the Hox-4.4 probe, but extends much more laterally.

Structure	Level	Pattern	Image	Note
TS20: embryo mesenchyme	Strong	Regionally restricted	7D -4.3	Strong Hox-4.3 labelling is seen in superficial mesenchyme at the base of the head, up to very lateral regions. This labelling is similar to that obtained with the Hox-4.4 probe, but extends much more laterally.
TS20: head mesenchyme	Strong	Regionally restricted	7D -4.3	Strong Hox-4.3 labelling is seen in superficial mesenchyme at the base of the head, up to very lateral regions. This labelling is similar to that obtained with the Hox-4.4 probe, but extends much more laterally.
TS20: humerus pre-cartilage condensation	Present	Regionally restricted	7D -4.3	Hox-4.3 labels the humeral head condensation.

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present		7E -4.3	Labelling occurs in fasciculated groups of cells in the very proximal area of the forelimb in the vicinity of the brachial blood vessels, identified as probable nerve trunks of the brachial plexus.

Structure	Level	Pattern	Image	Note
TS20: T10 vertebral cartilage condensation	Weak		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: L1 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: L2 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: L3 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: L4 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: L5 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: L6 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: S1 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: S2 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: S3 vertebral pre-cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: S4 vertebral pre-cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T11 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T12 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T13 vertebral cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: tail vertebral pre-cartilage condensation	Strong	Regionally restricted	6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: C1 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: C2 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: C3 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: C4 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: C5 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: C6 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: C7 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T1 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T2 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T3 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T4 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T5 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T6 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T7 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).
TS20: T8 vertebral cartilage condensation	Absent		6B -4.3	The Hox-4.3 expression domain is restricted along the anteroposterior axis in some ectoderm (the nervous system) and mesoderm derivatives (e.g. the prevertebrae, gut, or kidney).

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	8B -4.3	The cortex is labelled as well as parallel bundles of cells within the undifferentiated gonad surrounding the primitive sexual cords. Labelling is stronger in the metanephric tubules than in the stroma and stronger in the gonad than the thbules.
TS20: testis	Present	Regionally restricted	8B -4.3	The cortex is labelled as well as parallel bundles of cells within the undifferentiated gonad surrounding the primitive sexual cords. Labelling is stronger in the metanephric tubules than in the stroma and stronger in the gonad than the thbules.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Uniform	7A -4.3	Expression is present in the limb mesoderm, but axial mesoderm adjacent to the limb buds is not labelled.
TS15: hindlimb ridge mesenchyme	Present	Uniform	7A -4.3	Expression is present in the limb mesoderm, but axial mesoderm adjacent to the limb buds is not labelled.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from somatopleure	Present	Regionally restricted		Labelling is found specifically in the lateral somatopleure mesoderm posterior to the forelimb buds.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		7B -4.3	At this AP level, labelling is quite widespread in the embryonic body; the hybridization signal is far more intense within the hindlimb bud than in the adjacent axial tissues.
TS17: hindlimb bud	Strong		7B -4.3	At this AP level, labelling is quite widespread in the embryonic body; the hybridization signal is far more intense within the hindlimb bud than in the adjacent axial tissues.

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Present	Graded	8A	The mesonephric column is labelled, but a higher grain density is found along the ventral parts of the mesonephros, in areas from which the genital ridges develop.
TS17: mesonephric tubule	Strong		8A
TS17: nephric duct	Present		8A
TS17: intraembryonic coelom	Present	Regionally restricted	8A	Transcripts are detected in the coelomic epithelium and in cells immediately subjacent.