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Gene Expression Data
Assay Details
Assay
Reference: J:10735 Gubbay J, et al., Normal structure and expression of Zfy genes in XY female mice mutant in Tdy. Development. 1990 Jul;109(3):647-53
Assay type: RT-PCR
MGI Accession ID: MGI:1341465
Gene symbol: Zfy1
Gene name: zinc finger protein 1, Y-linked
Probe: Zfy1-pA, Zfy1-pB
Visualized with: Ethidium bromide
Assay notes: The results shown here represent amplification with 'nested' primers. The 'outer' primers used in a prior reaction are described in MGI:1341456. A complex breeding scheme is detailed in the reference. Littermates from two separate litters were analyzed.The control Hprt band was present in all samples.
Results
Image: 4 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (a) Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
1: XY male E12.5 TS20: testis Present Present Present 20 µg Not Specified X/Srydl1RlbMale
1: XY female E12.5 TS20: ovary Present Present Present 20 µg Not Specified X/Srydl1RlbFemale
1: XX female E12.5 TS20: ovary Absent Absent Absent 20 µg Not Specified Female
2: XY male E12.5 TS20: testis Present Present Present 20 µg Not Specified X/Srydl1RlbMale
2: XY female E12.5 TS20: ovary Present Present Present 20 µg Not Specified X/Srydl1RlbFemale
2: XX female E12.5 TS20: ovary Absent Absent Absent 20 µg Not Specified Female
No RNA Control
Notes:
(a) The three bands seen in the Xy tracks are due to alternative splicing in the 5' non-coding region of Zfy1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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