Probe:	TRP-2 probe-3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1B	Expression were detected in putative migrating cranial crest cells (located between the forebrain-midbrain junction and the eye, at the level of the midbrain-hindbrain junction, and posterior to the otic vesicle).
TS17: 1st branchial groove	Absent		1B
TS17: 2nd branchial groove	Absent		1B
TS17: 3rd branchial groove	Absent		1B
TS17: 4th branchial groove	Absent		1B
TS17: otocyst	Absent		1B

Structure	Level	Pattern	Image	Note
TS18: head mesenchyme	Present	Regionally restricted	1D	Expressing cells were present in head mesenchyme particularly in the region between the eye, forebrain, and nose.
TS18: branchial arch	Present	Regionally restricted	1D	Expression was detected in the posterior regions of the branchial arch.
TS18: 2nd branchial arch	Present	Regionally restricted	1D	Expression was detected in the posterior regions of the hyoid arch.
TS18: trunk mesenchyme	Present	Regionally restricted	1D	Expressing cells were observed from the first somites to the level of the hindlimb buds in the same locations as cells expressing Kit mRNA. No expression was detected in the lateral mesenchyme.
TS18: forelimb bud	Absent		1D
TS18: hindgut	Absent		1D
TS18: hindlimb bud	Absent		1D
TS18: neural tube	Absent		1D	No expression was detected in the dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS18: head mesenchyme	Present	Regionally restricted	1F	Expressing cells were localized in a stripe originating at the mid-to-hindbrain junction and extending towards the eye. No expression was detected in facial structures.
TS18: hindlimb bud	Absent		1F

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme	Present	Regionally restricted	2A	Expressing cells were distributed over the entire somite surface; no expressing cells were detected in the lateral mesenchyme.
TS19: forelimb bud	Absent		2A
TS19: hindlimb bud	Absent		2A

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted	2B	Expression was detected anterior and posterior to the eye, over the hindbrain, and around the otic vesicle.
TS19: 2nd branchial arch	Present	Regionally restricted	2B	Expression was detected in the posterior aspect of the hyoid arch.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Present	Regionally restricted	2D	Expressing cells were localized in the migrating staging area lateral to the neural tube, and just one or two somites more rostral cells were found over the somite lateral to the migrating staging area.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Present	Regionally restricted	2E	In progressively older (more rostral) somites, incrementally more cells were localized laterally, and fewer positive cells remained near the neural tube.
TS19: hindlimb bud	Present		2E	Authors report that expressing cells were first observed at the base of the hind limb bud.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Present	Regionally restricted	3A	Two groups of positive cells were detected: one group in the migrating staging area lateral to the neural tube, and the other localized to the dermatome.

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme	Present	Regionally restricted	3C	Expression was detected on the laminin-positive basement membrane.

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme	Present	Regionally restricted	3E	Expressing cells are evenly spread throughout the dermal mesenchyme at this axial level.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	4A	Expression was detected in regions around the ear.
TS20: trunk	Present	Regionally restricted	4A	Positive cells were detected between the neural tube and the lateral midline, and some cells as far ventral as the base of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	4B	Expression was detected in regions around the ear.
TS20: trunk	Present	Regionally restricted	4B	Positive cells were detected between the neural tube and the lateral midline, and some cells as far ventral as the base of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	4C	Expression was detected in regions around the ear, bordering the whisker fields, and over the nose.
TS20: hindlimb	Present	Regionally restricted	4C	Many positive cells were present on the posterior side of the hindlimb.
TS20: trunk	Present	Regionally restricted	4C	Positive cells were detected on the lateral sides of the tail.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	4D	Positive cells were detected between the neural tube and the lateral midline, and some cells as far ventral as the base of the forelimb bud.
TS20: trunk	Present	Regionally restricted	4D	No expression was detected on the ventral side of the body.

Structure	Level	Pattern	Image	Note
TS20: tail dermomyotome	Present	Spotted	6A

Structure	Level	Pattern	Image	Note
TS20: tail mesenchyme derived from neural crest	Present	Spotted	6B	Individual expressing cells were detected in the migrating staging area over a length of four to five segments.
TS20: brain	Present	Spotted	6B	Expression was detected in a few cells at the mid-hindbrain border.

Structure	Level	Pattern	Image	Note
TS20: tail dermomyotome	Present	Spotted	6C	A lower density of expressing cells were localized over the dermatome compared to wild-type embryos.
TS20: tail somite	Present	Spotted	6C	In contrast to Sl/Sl animals, many expressing cells were dispersed over the somites.
TS20: brain	Present	Spotted	6C	Expression was detected in a few cells at the mid-hindbrain border.
TS20: tail mesenchyme derived from neural crest	Present	Spotted	6C	Individual expressing cells were detected in the migrating staging area over a length of four to five segments.

Structure	Level	Pattern	Image	Note
TS20: tail mesenchyme derived from neural crest	Present	Spotted	6D	Individual expressing cells were detected in the migrating staging area over a length of four to five segments. Expression pattern in the tail region was similar to the pattern in Sl/Sl mutants.

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent